|
Thèses |
|
1
|
-
Bruna Mendonça Lima
-
Development and optimization of hybrid nanossystems for resveratrol transport through a membrane with porosity similar to the blood-brain barrier
-
Leader : LUCIANO PAULINO DA SILVA
-
MEMBRES DE LA BANQUE :
-
Juliana Junqueira Pinelli
-
JOAO PAULO FIGUEIRO LONGO
-
LUCIANO PAULINO DA SILVA
-
VERA LÚCIA PERUSSI POLEZ
-
Data: 28 févr. 2023
-
-
Afficher le Résumé
-
The central nervous system (CNS) is protected by a semipermeable dynamic structure, called the blood-brain barrier (BBB), which efficiently shields this area from possible changes caused by harmful agents from the environment. In this sense, liposomal nanosystems present gains in performance and functionality, with a view to facilitating the passage of drugs through this barrier. Among the nutraceuticals that can be therapeutically used, resveratrol (RES) is a very promising flavonoid for acting as an anti-inflammatory and antioxidant agent. In the present work, a liposomal phosphatidylcholine nanosystem was made associated with poloxamer (pluronic F127) micelles for RES encapsulation. Each step of the experiments (production of empty and RES-filled pluronic F127 micelles, empty and RES-filled liposomal nanosystems, empty and extruded extruded liposomal nanosystems with RES) were repeated at least three times to verify their reproducibility, in addition to having the diameter values hydrodynamic (Z-Ave and percentage numbers) and polydispersion (PdI) measurements accounted for. About the Z-Ave, each group obtained different results including statistical difference between then. Therefore was necessary to review the hydrodynamic diameter in numbers in order to solve this issue. The PdI showed the importance of the extrusion method to obtain a more monodisperse solution (close to 0,2). Afterwards, the encapsulation rate was evaluated in the three experiments (batches) of extruded liposomal nanosystems with RES with an encapsulation rate of 63.94%, 89.10% and 100%. Then, a new liposomal extruded structure was constructed with RES and subjected to passage through a membrane with porosity compatible with that of the BBB. This nanosystem was able to perform diapedesis and maintain its size close to before passage. The liposomal nanosystem proved to be effective for RES encapsulation with an encapsulation rate compatible with that of the reviewed literature when compared to other nanosystem types. The results confirm the initial assumptions of high efficiency of the encapsulation capacity of the in vitro analyses and in vivo future studies will be necessary to verify the functionality of RES delivery in animal models.
|
|
2
|
-
Bruno Zschaber Mavignier de Castro
-
Characterization of soil traces aiming at the production of forensic material evidence
-
Leader : MARCELO HENRIQUE SOUSA
-
MEMBRES DE LA BANQUE :
-
MARCELO HENRIQUE SOUSA
-
JORLANDIO FRANCISCO FELIX
-
JULIANO ALEXANDRE CHAKER
-
EDUARDO JOSÉ JUCÁ MALLMANN
-
Data: 30 juin 2023
-
-
Afficher le Résumé
-
Soil is a mixture of organic and inorganic matter whose components, including clay, contain specific individualizing characteristics. In the forensic context, soil traces are important evidence, since they often adhere to individuals and objects associated with criminal actions. Based on the analysis of said traces, it is possible to link a suspect to a crime scene or to rule out such a link. In the present study, the hypothesis was raised that soil samples collected from different locations can be differentiated from each other based on the analysis of their clay fraction alone. To test the presented hypothesis, 16 (sixteen) samples of Oxisol and 16 (sixteen) samples of Cambisol were collected from the superficial layer (depth between 0 and 10 cm), in transects of areas with the same geology and geomorphology. In the case of the Oxisol, collections were carried out 2 m, 10 m, 100 m and 1,000 m apart, totaling a total distance of 4,446 m and, in the case of the Cambisol, the samples were collected every 2 m, 10 m, 100 m and 300 m. m apart, totaling 1,646 m. Clay fractions were extracted from soil samples and analyzed for crystallography by X-ray diffraction (Synchotron) and for Zeta potential. The dataset was subjected to multivariate analysis and the results were effective in distinguishing samples from the two types of soil. When samples from the same soil were analyzed among themselves, the results varied according to the technique used. The forensic analysis of soil samples is therefore a promising field, since the clay portion present in said samples can be useful even when routine analytical techniques are used.
|
|
3
|
-
ALICE LIMA ROSA MENDES
-
EVALUATION OF THE APPLICATION OF NANOSTRUCTURED NARINGENIN IN AGRICULTURE
-
Leader : LIVIA CRISTINA LIRA DE SA BARRETO
-
MEMBRES DE LA BANQUE :
-
LIVIA CRISTINA LIRA DE SA BARRETO
-
MARCILIO SERGIO SOARES DA CUNHA FILHO
-
JAIRELDA SOUSA RODRIGUES
-
TALITA FARAJ FARIA
-
Data: 6 juil. 2023
-
-
Afficher le Résumé
-
Objective: To develop and characterize nanosystems containing Naringenin as an effective alternative for controlling ticks in livestock and mites in agriculture. Methodology: The analysis of nano formulations (Nanosystem 1, Nanosystem 2 and Nanosystem 3). The positive control was represented by drug formulations, while the negative control was represented by nano formulations without the active principle. The pH, the Zeta Potential, the PDI and the droplet size were evaluated for the characterization of the nano formulations in order to verify their acaricidal activity and toxicity to seed germination as a therapeutic alternative for the control of ticks through a test dipped in washing and dipped in engorged females. Results and Discussion: The addition of components to the nano formulations resulted in the variation of the size, PDI, Zeta Potential and pH of the nanoparticles. This result is expected, since alterations in the structure of nanoparticles cause the rearrangement and variation of its parameters. The nanoformulation Nanosistema 1 NAR, Nanosistema 2 NAR, Nanosistema 3 NAR, was known to be more effective than distilled water and Amitraz (positive control) in controlling R. microplus, suggesting that they may be good options for positive control for the adult immersion test. The submerged test on larvae showed that the distilled water control did not have a deleterious effect on the larvae, while the positive control Triatox (Amitraz) showed 100% mortality, according to FAO standards (95%). The nanoformulations containing Nanosystem 1 NAR and Nanosystem 2 NAR were effective, as they generated 95% and 96% of mortality, respectively. These results suggest that the permeation of the active principle is more efficient and effective, as they allow the drug to be deposited on the skin, generating an occlusive effect, which prevents gas exchange in the mite cells. Tests appreciated that Nanosystem 3 NAR had an increase in germination by 68%. The application of nanoformulations Nanosistema 1 NAR and Nanosistema 2 NAR increased seed germination by more than 75%, exceeding the requirements of Normative Instruction No. 42 of 2019, making it effective as well as negative water control. Conclusion: Pathogenic studies that nano formulations containing naringenin as an active principle can reach the appropriate physicochemical requirements and offer effectiveness for the control of acaricide resistance in relation to teleogynes and bovine tick larvae. Lettuce seeds were also tested, revealing that the nanosystems were effective and safe in the physicochemical and biological quality of the nanoformulations. This formulation opens up opportunities for the development of new formulations and dosage forms.
|
|
4
|
-
Louiz Gonçalves dos Santos
-
Analysis of molecular changes in testes submitted to nanoparticle-mediated photohyperthermia as a method of inducing infertility
-
Leader : CAROLINA MADEIRA LUCCI
-
MEMBRES DE LA BANQUE :
-
ANDREA QUEIROZ MARANHAO
-
CAROLINA MADEIRA LUCCI
-
JULIANA LIS MENDES DE BRITO
-
MAURÍCIO MACHAIM FRANCO
-
Data: 26 juil. 2023
-
-
Afficher le Résumé
-
The objective of the present work was to investigate molecular alterations caused by the procedure of photohyperthermia mediated by nanoparticles (FHT) applied to testicles of Wistar rats. The animals were treated and divided according to the moment of euthanasia: 24 hours, 72 hours and 7 days after the procedure. The testicles were measured, weighed and segmented, one part destined for the evaluation of molecular markers and one part for histopathological evaluation. For the analysis of molecular markers, the extraction of total RNA from the samples was performed and the expression of the genes Mtrf1, Trf2, CLDN11 and StAR was analyzed, normalized by the GAPDH and B-actin genes. The Mtrf1 and Trf2 genes showed a significant reduction in expression after treatment, mainly in the 24h and 72h groups, remaining low in the 7-day group. The CLDN11 gene did not show statistical difference, however it is possible to observe an increasing tendency, more evident in the animals analyzed 7 days after FHT. The StAR gene had higher expression in the testes of treated animals 24h after treatment, even with variation between animals, and reduced after 72h and 7 days. Histopathological analyzes showed slight alterations in the 24h and 72h groups, however, the beginning of vacuolation and exfoliated cells in the lumen of some tubules were already visible. In the 7-day samples, the alterations were more pronounced, with greater vacuolization, reduction in the size of the tubules and extensive coagulative necrosis. The work showed that testicular FHT treatment affects the expression of genes that may be involved in ROS production or antioxidant protection pathways, and histopathological analyzes showed the damage caused by the treatment, corroborating that the procedure causes irreversible damage to testicular function. It is still necessary to analyze the expression of other genes to better identify the regulatory pathways.
|
|
5
|
-
Julia Augusto Vieira
-
Development of a nanobiosensor of gold nanoparticles (AuNPs) functionalized with anti-SARS-CoV-2 glycoprotein antibodies and testing in volunteer patients.
-
Leader : RICARDO BENTES DE AZEVEDO
-
MEMBRES DE LA BANQUE :
-
Glêndara Aparecida de Souza Martins
-
PATRICIA BENTO DA SILVA
-
RICARDO BENTES DE AZEVEDO
-
SACHA BRAUN CHAVES
-
Data: 28 juil. 2023
-
-
Afficher le Résumé
-
The current COVID-19 pandemic, caused by the SARS-CoV-2 virus, comprises an infectious disease. Coronaviruses (CoVs), which are part of a viral family, are recognized for causing a variety of respiratory diseases in humans, ranging from the common cold to rarer and more severe conditions such as Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). This information is recorded by the World Health Organization panel. As far as transmission is concerned, respiratory droplets are the primary means of contamination, allowing SARS-CoV-2 to be transmitted from an infected person to a healthy person through physical contact with the sick patient or by coming into contact with their belongings. Coronaviruses (CoVs) have the ability to remain on surfaces for several days, which may explain the case of SARS-CoV-2 and represent a prolonged risk of infection. Nanotechnology has found extensive application in various fields of study, including medicine, biolabeling, and agriculture. Nowadays, this technology has been employed in different domains of medical science, such as targeted drug delivery, cancer diagnosis, and the development of agents against viral, bacterial, and fungal infections. Gold nanoparticles (AuNPs) exhibit characteristics that include the ability to provide a stable surface for the immobilization of biomolecules, which is highly useful in the preparation of biosensors. They are considered the most stable metallic nanoparticles due to their optical, electronic, and catalytic properties, as well as having high biocompatibility and an improved rate of electron transfer. For these reasons, AuNPs have found wide applications in various types of biosensors. In the present study, the development of a nanobiosensor was conducted using AuNPs that were functionalized with antibodies specific for the SARS-CoV-2 glycoprotein. The main goal of this nanobiosensor was to enable highly specific detection of the SARS-CoV-2 virus. The characterization results of the naked and functionalized AuNP50 and AuNP100 showed that the nanoparticles were stable (hydrodynamic diameter and polydispersity index). The functionalized nanoparticles showed good performance regarding binding on SARS-CoV-2 viruses. Finally, when the functionalized AuNP100 were put in contact with the samples from positive patients, there was a change in the size of the nanoparticles verified by dynamic light scattering (DLS) technique.
|
|
6
|
-
Débora Pires Porto Barbosa
-
" In vitro skin penetration study of iron oxide nanocarriers with different coatings ".
-
Leader : MARIA APARECIDA GODOY SOLER PAJANIAN
-
MEMBRES DE LA BANQUE :
-
Elton Brito Ribeiro
-
JOSE JOAQUIN LINARES LEON
-
MARIA APARECIDA GODOY SOLER PAJANIAN
-
PAULO EDUARDO NARCIZO DE SOUZA
-
Data: 29 août 2023
-
-
Afficher le Résumé
-
The cutaneous penetration of different iron oxide nanocarriers has been investigated to evaluate its application as topical administration of drugs. The nanoparticles (NPs) were synthesized via chemical coprecipitation in basic aqueous medium and functionalized with three different surfactants, lauric acid (anionic), dodecylamine (cationic) and Pluronic F127 (non-ionic), resulting in stable aqueous coloidal dispersions. The synthesized nanocarriers were characterized by dynamic light scattering, transmission electron microscopy, infrared and Raman spectroscopy. The NPs presented mean diameter of about 6.5 nm, while the hydrodynamic diameter ranged from 130 to 230 nm. Raman spectra showed that the oxidation of the magnetite particles to maghemite was effective, while the analysis of the Infrared Spectra indicated the presence of each coating layers on the 3 nanocarriers. In vitro skin penetration studies of the 3 nanosystems, was conducted using pig ear skin as a model for human skin through the modified Saarbrüecken method. The permeation was conducted during 12, 24 and 36 hours. The concentration of iron was determined through ferromagnetic resonance measurements following permeation. The concentration of iron in the stratum corneum and the hair follicle, for each nanonocarries was obtained. It was not observed the presence of iron in the remaining skin. These results indicate that all the synthesized nanocarriers present potential for topical application of drugs, allowing a local treatment, without reaching the bloodstream.
|
|
7
|
-
Aline Beatriz da Silva Santos
-
Development of Gold/Pluronic Nanocomposites for Biological Applications
-
Leader : MARIA APARECIDA GODOY SOLER PAJANIAN
-
MEMBRES DE LA BANQUE :
-
ELAINE ROSE MAIA
-
LUANA ALVES DE FREITAS AFIUNE
-
MARIA APARECIDA GODOY SOLER PAJANIAN
-
PAULO EDUARDO NARCIZO DE SOUZA
-
Data: 30 août 2023
-
-
Afficher le Résumé
-
The objective of this work is the preparation, characterization and biomedical application of nanocomposites formed by gold nanoparticles and Pluronic® F-127. The nanocomposites were synthesized from the mixture of Pluronic® triblock copolymer solutions varying the concentrations in (0.5; 2.0 mmol.L-1), and chloroauric acid (HAuCl4) (0.1; 2. 0, 3.0, 4.0 mmol.L-1), in a UV radiation chamber (254nm), at room temperature. In this reaction, the copolymer acts in the reduction of gold ions and stabilization of gold nanoparticles, forming the AuNP-PLU colloidal nanocomposite. The ultraviolet-visible spectroscopy analysis shows that the surface plasmonic resonance band of the nanocomposites is located between 525 to 529 nm. The micrographs of the nanocomposites obtained show gold nanoparticles with a spherical, triangular or cylindrical shape, with an average diameter of 5 to 44 nm. The average hydrodynamic (DH) size obtained by dynamic light scattering ranged from 31 to 68 nm. For the biological application experiments, nanocomposites 2.0AuNP-PLU:2.0 and 2.0AuNP-PLU:0.5 were chosen, as they presented greater reproducibility and stability of the physical-chemical characteristics after synthesis. These nanocomposites were used in the photochemical test with 1,3-diphenylisobenzofuran (DPBF), in which the formation of reactive oxygen species (ROS) was observed. To carry out this test, the nanoparticles were associated with a photosensitizer, aluminum phthalocyanine chloride (AlClPc), in concentrations of 0.1 to 5.0 µg. After mixing with the photosensitizer, the nanocomposites were characterized by ultraviolet-visible spectroscopy, in which it was possible to observe a new peak at 680 nm coming from the photosensitizer. The new average hydrodynamic (DH) size of the nanocomposites with the photosensitizer was from 31 to 73 nm. The characterization was performed by Raman spectroscopy and by infrared spectroscopy (FTIR-ATR), in which changes were observed in the spectra of the nanocomposites due to the bonds between the ether groups of the pluronic with the nitrogenous groups of the aluminum phthalocyanine chloride. In another approach, the 2.0AuNP-PLU:2.0 nanocomposite was used to study the maternal and fetal toxicological effects of its administration during pregnancy in rats and fetuses. The results showed that the nanocomposite is safe for both the mother and the fetus at a dose of 137.5 μg/kg. Therefore, the administration of the nanocomposite did not result in changes in fetal development, nor changes greater than 5% in the observed organs. The same 2.0AuNP-PLU:2.0 nanocomposite was investigated as a contrast agent in computed tomography. The results showed that the contrast agent is comparable to the commercially used contrast (Optiray® 320) for the same beam energy.
|
|
8
|
-
Samuel Silva Morais
-
"DEVELOPMENT OF PEQUI OIL NANOEMULSIONS (CARYOCAR BRASILIENSE) OBTAINED BY DIFFERENT EXTRACTION METHODS AND EVALUATION OF THEIR EFFECTS ON THE ACTIVITY OF RAW 264.7 MACROPHAGES IN VITRO".
-
Leader : GRAZIELLA ANSELMO JOANITTI
-
MEMBRES DE LA BANQUE :
-
GRAZIELLA ANSELMO JOANITTI
-
LUIS ALEXANDRE MUEHLMANN
-
MONICA PEREIRA GARCIA
-
BRENO NORONHA MATOS
-
Data: 8 déc. 2023
-
-
Afficher le Résumé
-
Inflammation is one of the body's defense processes and occurs in response to infections caused by microorganisms or tissue damage. Pequi oil extracted from Caryocar brasiliense found in the Cerrado region is popularly used for its anti-inflammatory and antioxidant properties due to the presence of fatty acids and carotenoids. Nanotechnology serves as a tool to overcome barriers in the administration of hydrophobic compounds. This study aimed to produce and evaluate the physico-chemical properties and cytotoxicity of nanoemulsions based in pequi oil (PeNE) obtained through different extraction methods: cold pressing, solvent (acetone), and boiling (artisanal method). The pequi oils were evaluated based on their lipid profile, acidity and peroxide values to ensure their quality after extraction processes. The nanoemulsions were formulated with pequi oil, egg lecithin (2:1 w/w), and PEG40 (2%) and dispersed in PBS by ultrasonication. The samples were evaluated using Dynamic Light Scattering (DLS) for 120 days at 4°C and 25°C to assess their stability, as well as pH and stress conditions (serial dilution from 0 to 1/128 and pH variation between 3-11). For cytotoxicity assays, RAW 264.7 macrophages were treated with different PeNE and had their viability analyzed by MTT assay. The pequi oils maintained high purity levels according to the evaluated indices. PeNE obtained with oil obtained by different extraction methods showed similar hydrodynamic diameter <100 nm, PDI <0.250, zeta potential between -1.5 and -2.5 mV, pH between 6 and 7, and remained stable during the dilution test without significant variation and pH variation tests showed zeta potential changes in the most acidic pH the nanoemulsions remained neutral (3) near to 0 mV the most basic (11) got negative near to -10 to -15 mV. The MTT assay indicated that the concentration of 360 µg/mL of different PeNE and free oils did not show significant cytotoxicity to the cells after 24 hours, with cellular viability >80%. The nanoemulsions formulated from pequi oil obtained by different extraction methods remained stable, and the extraction methods did not lead to differences between the samples in the evaluated parameters. These nanoemulsions showed promise for application with macrophages, which are crucial cells in the inflammatory process, demonstrating biocompatibility for future evaluations.
|
|
9
|
-
PEDRO ANDRÉS GRANADOS MUÑOZ
-
ESTUDOS DE PRÉ-FORMULAÇÃO PARA O DESENVOLVIMENTO DE UM FILME ORAL MUCOADESIVO CONTENDO TRIANCINOLONA ACETONIDA POR HOT-MELT EXTRUSION PARA O TRATAMENTO DE ESTOMATITE AFTOSA RECORRENTE
-
Leader : MARCILIO SERGIO SOARES DA CUNHA FILHO
-
MEMBRES DE LA BANQUE :
-
MARCILIO SERGIO SOARES DA CUNHA FILHO
-
IDEJAN PADILHA GROSS
-
ADRIANA MARCELA NIETO MUNOZ
-
GEISA NASCIMENTO BARBALHO
-
Data: 19 déc. 2023
-
-
Afficher le Résumé
-
Recurrent aphthous stomatitis (RAS) is the most widespread oral disease, affecting up to 25% of the global population. Triamcinolone acetonide (TA) has been widely used in the topical treatment of RAS, but current pharmaceutical forms are not efficient for this purpose, so there is a significant need to develop an improved drug administration system. This work proposes to obtain a TA-containing amorphous solid dispersion (ASD) processed by hotmelt extrusion (HME) in the form of a mucoadhesive oral film as an alternative to the RAS treatment. To this end, a detailed preformulation protocol was carried out using theoretical and experimental approaches, leading to the selection of four polymers and a plasticizer. From the Hansen solubility parameters (HSPs) the miscibility of the drug with the polymers was predicted as PVA > EUD > PVPVA > HPMCAS, while thermal analysis (DSC) showed an experimental miscibility, based on the percentage of drug in amorphous state as follows: EUD (81.07%) > PVA (67.55%) > PVPVA (45.57%) > HPMCAS (8.46%). The DTG curves showed instability problems with PVA, PVPVA and HMPCAS, as well as the plasticizer (TEC) evaporation. FTIR spectra confirm the instability and exhibit the maintenance of TEC within the HME processing temperature. The TA-EUD-TEC mixture (10:60:30, w/w) was successfully extruded forming an ASD in the form of a mucoadhesive oral film with a thickness of 0.69 ± 0.06 mm, moderate adhesive potential (0.216N ± 0.031), and 99.6% of drug content. The physicochemical characterization was carried out by DSC, TGA, FTIR and SEM. The results suggest partial evaporation of the liquid plasticizer and formation of heat-dependent anhydrides derived from the polymer chain, thus, the stability of the drug within the polymer matrix must be carefully evaluated. Notably, the pre-plasticization procedure was a key parameter for the success of the HME process.
|
|
|
Thèses |
|
1
|
-
Andréia Camargo Pinheiro
-
EVALUATION OF THE ANTI-INFLAMMATORY EFFECT OF PEQUI OIL-BASED NANOEMULSION (Caryocar brasiliense) ON in vitro MACROPHAGES AND in vivo EDEMA MODEL.
-
Leader : GRAZIELLA ANSELMO JOANITTI
-
MEMBRES DE LA BANQUE :
-
GRAZIELLA ANSELMO JOANITTI
-
ANAMELIA LORENZETTI BOCCA
-
MARCELO HENRIQUE SOUSA
-
ANDREANNE GOMES VASCONCELOS
-
PATRICIA BENTO DA SILVA
-
Data: 24 mars 2023
-
-
Afficher le Résumé
-
Inflammation is a set of changes that occur in the body and begin immediately after a stimulus, remaining associated with various causes, ranging from abandonment to stress or dysfunction related to emotional, immunological or genetic factors. Chronic inflammation is related to several diseases, being related to the evolution of noncommunicable chronic diseases, such as obesity, diabetes and cancer, which are now the main causes of mortality in the world, a phenomenon that has contributed to a significant increase in the medical demand for inflammatory cases. Anti-inflammatory drugs are among the most prescribed and consumed drugs in the world, being used since 1897, with the production of aspirin. Despite being widely observed over these years, they still have an important limitation of use, which is associated with prolonged time and consequent adverse effects. New research has raised an alarming concern in relation to new discoveries relating the use of anti-inflammatories to gastrointestinal, cardiovascular, hepatic, renal, respiratory and pulmonary complications, which raises the need for new drugs with effective anti-inflammatory action and that bring safety for long-term use. In this regard, Brazilian biodiversity represents a rich source of release of bioactives that can contribute both to the resolution and prevention of inflammatory processes. Pequi oil (Caryocar brasiliense), a plant native to the Brazilian Cerrado, consists mainly of fatty acids and carotenoids that give it several biological properties, including an antiinflammatory effect. However, the hydrophobicity of these molecules represents a limit to their administration. Therefore, the pequi oil was nanostructured in the form of a nanoemulsion (PeNE) as a way to improve the transport and delivery of the oil to the cells. In this work, investigations were conducted in 3 chapters: (1) systematic review evaluating the anti-inflammatory potential of pequi oil in its free or nanostructured form; (2) synthesis and characterization of PeNE, evaluation of cytotoxicity in macrophages from the bone marrow of rats differentiated by L929, in vitro, on cell viability and evaluation of the anti-inflammatory potential on these cells regarding the modulation of inflammatory mediators and morphological activation changes; (3) synthesis, characterization and evaluation of the anti-inflammatory and nociceptive effect of PeNE by model of edema induced by carrageenan, in addition to toxicity evaluation through clinical, biochemical, hematological and histopathological analyzes in male rats Rattus novergicus, In vivo, in addition to evaluation of cytotoxicity, DNA fragmentation and incorporation of NePE in J774-1 macrophage cells, in vitro. The results showed that there are several studies indicating the anti-inflammatory activity of pequi oil, however, so far there is no systematic review involving these data and that the literature points to the interaction of pequi oil with mechanisms of the inflammatory process through the modulation of pro and anti-inflammatory mediators. In addition, success was achieved in obtaining stable PeNE and in accordance with the proposal to be used as a nanophytotherapy, with dose-dependent cytotoxic activity on macrophages, biocompatibility, anti-inflammatory and anti-nociceptive activity in in vitro and in vivo models. Thus, the use of nanostructured pequi oil as an alternative or complement to conventional anti-inflammatory drugs is promising and needs attention for future research studies to better understand its anti-inflammatory and anti-nociceptive potential.
|
|
2
|
-
Fellipy Samuel Rocha
-
"Study fo the copper(II)phthalocyanine tetrasulfonic acid tetrasodium salt (CuPcSO4) association with TiO2/Graphene nanocomposite with a photocatalytic overview".
-
Leader : CLAURE NAIN LUNARDI GOMES
-
MEMBRES DE LA BANQUE :
-
CLAURE NAIN LUNARDI GOMES
-
ELTON CLEMENTINO DA SILVA
-
VIVIAN DA SILVA SANTOS
-
ALISSON MENDES RODRIGUES
-
EDUARDO HENRIQUE DUARTE
-
Data: 19 juil. 2023
-
-
Afficher le Résumé
-
Titanium dioxide TiO2 is one of the primary compounds for photocatalysis applications. Even though it has been extensively studied, there is still room to improve its photocatalytic properties and photoabsorption. TiO2 presents photocatalytic activity under uv-vis light; however, only 5% of solar energy is present in this region, while 45% comprises visible electromagnetic radiation. Phthalocyanines are studied due to their capacity to interact with visible light, and the copper(II)phthalocyanine tetrasulfonic acid tetrasodium salt (CuPcSO4) was associated with TiO2 and graphene (GR) attached to TiO2. TiO2-GR adsorbed CuPcSO4, and the photochemical behavior was studied. TiO2- GR adsorbed 26% more CuPcSO4 than TiO2 alone. The incorporation of CuPcSO4 onto the surface of TiO2-GR generates a bathochromic shift of the typical graphene response, which indicates that the adsorption of CuPcSO4 reduces the TiO2-GR bandgap. The CuPcSO4 presents a clearer separation of the Q band when adsorbed onto the TiO2 surface, and the Qy band becomes the primary one. The increase and shift in the Qy band indicate a stronger response for low-energy light sources for the phthalocyanine, hence improving the light-harvesting capability of the compound. TiO2-CuPcSO4 and TiO2-GR-CuPcSO4 interact with amphotericin B (amB), demonstrated by the drug absorbance's hypochromic shift, indicating a higher aggregation state. The energy and chemical compounds consumed during the synthesis contribute to the environmental impacts. It is important to elucidate the practical and economic advantages of functionalizing the material. A simplified life cycle analysis (LCA) was conducted with 1kg of catalyst production as a functional unit. The compounds with 30 wt% graphene presented 80% more impact in some categories. An applied engineering concept is added to visualize the viability of developing a pilot model for wastewater treatment. The hydraulic system idea was adopted with an effluent flow of 1 L/min to facilitate calculations. Heterogeneous catalysts are a promising process for environmental remediation.
|
|
3
|
-
MELISSA SILVA MONTEIRO DE MENDONÇA
-
"In vitro and in vivo evaluation of antitumor therapy with radiofrequency and gold and graphene nanostructures in a Walker 256 tumor model".
-
Leader : SONIA NAIR BAO
-
MEMBRES DE LA BANQUE :
-
BRUNA CANDIDO GUIDO
-
CRISTIANO JACQUES MIOSSO RODRIGUES MENDES
-
MARCELLA LEMOS BRETTAS CARNEIRO
-
MARCIA CRISTINA OLIVEIRA DA ROCHA
-
SONIA NAIR BAO
-
Data: 21 déc. 2023
-
-
Afficher le Résumé
-
Estimates from GLOBOCAN 2020, produced by the International Agency for Research on Cancer (IARC), reported 9.9 million deaths from 36 types of cancers across 185 countries, accounting for 1 in every 6 deaths due to cancer. The predicted cancer incidence for the same year (excluding nonmelanoma skin cancer) was 19.3 million cases. In light of this global scenario, minimally invasive adjunct treatments such as radiofrequency ablation for early-stage tumors emerge as eligible options for reducing this statistic. Radiofrequency Ablation (RFA) is a therapeutic approach designed to induce programmed tissue injury through resistive heating safely and efficiently. Despite being considered a safe and effective technique, RFA has limitations, including a restricted ablation area of 3 cm and recurrence in tumors of 3 to 5 cm. This study aims to promote a combined therapy of RF obtained by an ablation device named SOFIA®, developed by the Laboratory of Biomedical Engineering (LaB), with the conjugated application of a gold and graphene nanostructure in the form of the graphene oxidepolyethyleneimine-gold composite (GO-PEI-Au), produced by the Laboratory of Research in Polymers and Nanomaterials (LabPoln), both from the University of Brasília. In vitro assays were conducted with the LLC WRC 256 (Walker 256) cell line, ex vivo with pig liver within 2 hours post mortem, and in vivo with Wistar rats carrying the Walker 256 tumor model. Therapeutic efficacy was assessed through in vitro analyses of cell viability, ultrastructure, and tumorigenesis; ex vivo for macroscopic evaluation of the ablation area, temperature increase, and impedance variation; and in vivo with clinical, histopathological, and immunohistochemical analysis. In the in vitro assays, a promising remission of the LLC WRC 256 (Walker 256) cell line was observed with the combined therapy of the nanocomposite and RF application, along with the presence of GO-PEI-Au in the cellular nucleus up to 6 hours posttreatment and a significant reduction in colony formation. The ex vivo assay highlighted the importance of conjugating graphene oxide with polyethyleneimine (PEI) to reduce impedance during the RFA procedure. In the in vivo survival assay, an earlier tumor reduction and enhanced nanocomposite uptake by the tumor were observed in the combined therapy group compared to the RF or GO-PEI-Au treatment groups alone. The in vivo assay showed an early tumor remission and increased nanocomposite uptake in the presence of radiofrequency, as well as lower BCL2 activity by the tumor post-ablation, contrasting with RF therapy that indicated a synergy of BCL2 and p53 protein coexpression, suggesting an indicative overexpression of recurrence.
|
|