Banca de QUALIFICAÇÃO: Philippe de Castro Lins

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : Philippe de Castro Lins
DATE: 22/04/2024
TIME: 14:00
LOCAL: Plataforma Teams
TITLE:

METAGENOMIC BIOPROSPECTION WITH BIOTECHNOLOGICAL POTENTIAL OF CERRADO SAMPLES

KEY WORDS:

metagenomics, dioxygenase, beta-lactamase, microbial resistance

PAGES: 60
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Bacteria have the ability to adapt to stressful situations, expressing specific genes that confer resistance. However, in addition to the genes responsible for the survival of the microorganism, it is possible that other genes assume this physiological role in the absence of this specific class. The complexity of microbial physiology is still not fully understood. Certain genes, which express enzymes responsible for the metabolism of aromatic compounds, can adapt to stress conditions and confer resistance to antibiotics. In this study, two samples from the Cerrado Biome were studied, identifying enzymes with biotechnological interest, the first sample being the soil and the second intestine of termite from the Cerrado. For the Cerrado soil sample, a dioxygenase was identified, which conferred resistance to a strain of bacteria expressing the antibiotic carbenicillin, a beta-lactam. In addition, new beta-lactamase genes were isolated from the termite intestine. In the study, a new dioxygenase was characterized through metagenomic isolation of Cerrado soil. The enzyme showed optimal activity at pH 7 and temperature of 30°C, using iron ions as a cofactor for substrate cleavage. The catalytic dynamics of CRB2 demonstrated values of Vmax = 0.02281 µM/min and KM = 97.6. The three- dimensional structure revealed substrate binding to the termite domain, where the active site is located. The analyzed substrates interact directly with the iron ion, coordinated by three histidine residues. Alternatively, modifying the iron ion charge can change the binding between the active site and substrates. For the termite intestine sample, genome annotation also revealed genes involved in hemicellulose metabolism, glycerol production and a possible β-lactamase gene. Taxonomy based on scaffold genes from the metgenome revealed that members of Acholeplasma are more abundant in the intestinal segment P3 than in P1. Within that of Acholeplasma, the computational analysis revealed beta-lactamases not yet described in the database. Metagenomics allows analyzing the biotechnological potential of several organisms simultaneously, based on sequence analysis and functional activity. Currently, there is a demand for enzymes with biotechnological activities of interest

COMMITTEE MEMBERS:
Presidente - 1208515 - ANDREA QUEIROZ MARANHAO
Externo à Instituição - FABYANO ALVARES CARDOSO LOPES - UFT
Externa ao Programa - 3333449 - JANICE LISBOA DE MARCO - nullInterno - 2644635 - ROBERT NEIL GERARD MILLER