Banca de DEFESA: Erica Cristina Silva Rego

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Erica Cristina Silva Rego
DATE: 28/02/2023
TIME: 08:00
LOCAL: Auditorio do IB
TITLE:

Characterization of microRNAs in Musa acuminata induced during interaction with Pseudocercospora musae

KEY WORDS:

Sigatoka leaf spot, Musa, microRNAs, biotic stress, Illumina

PAGES: 72
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Endogenous microRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level by cleavage or repression of mRNA translation. MiRNAs in plants regulate diverse cellular processes, including defense responses to biotic stresses. Banana (Musa spp.), a monocotyledonous crop cultivated throughout tropical regions, is susceptible to numerous diseases due to sterility and a narrow genetic background. Pseudocercospora musae, the causal agent of Sigatoka leaf spot disease, is an important fungal pathogen of banana, causing losses due to reduction in functional leaf area. Here, leaf RNA samples were extracted from Musa acuminata subsp. burmannicoides, var. Calcutta 4 (resistant), at 3 and 12 days after inoculation (DAI) with conidiospores. Following small RNA library construction, samples were sequenced using lllumina HiSeq 2500 technology. High quality sequences were mapped against the M. acuminata ssp. malaccensis var. Pahang reference genome and plant miRNAs were predicted using the programs Mireap and ShortStack. A total of 228 conserved miRNAs belonging to 30 miR-families were identified, together with 22 predicted novel miRNAs. At 3DAI, 48 miRNAs from 25 miR-families plus two novel miRNAs were significantly differentially expressed between inoculated and control samples. At 12DAI, 31 miRNAs from 18 different miR-families and two novel miRNAs regulated after inoculation. Potential host gene targets of miRNAs were predicted using TargetFinder. The characterization of miRNAs in M. acuminata and their role in gene expression modulation during interaction with P. musae provides resources for the development of efficient methods for control of Sigatoka leaf spot disease.

BANKING MEMBERS:
Externa à Instituição - Lucilia Helena Marcellino - EMBRAPA
Interna - 2222088 - ELIANE FERREIRA NORONHA
Externa à Instituição - MARÍLIA DE CASTRO RODRIGUES PAPPAS - EMBRAPA
Interno - 1661623 - RICARDO HENRIQUE KRUGER
Presidente - 2644635 - ROBERT NEIL GERARD MILLER