Banca de DEFESA: Vitoria Pinheiro Balestrini

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Vitoria Pinheiro Balestrini
DATE: 27/02/2023
TIME: 14:00
LOCAL: bloco K do IB, Primeiro Andar
TITLE:

Analysis of metagenome-assembled genomes from a lignin-enriched microbial community.

KEY WORDS:

Lignin degradation, Metabolism, Bacteria, Metagenomics, Community enrichment, Soil.

PAGES: 1
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The current concern for more ecologically sustainable processes has led to a demand for studies on the valuation of a material that commonly has its potential wasted, in this case the lignin present in plant biomass. The enzymatic recalcitrance caused by the complex and amorphous structure ends up being one of the limiting steps in the lignin recovery process and, therefore, this compound is usually burned to generate energy in the industries. Despite of this, microorganisms such as fungi and bacteria are known to be able to circumvent this recalcitrance through their enzymes. The deconstruction of lignin by bacteria is a recent branch of research and represents several advantages against fungi. In this study, the microbial diversity of three enriched consortia microorganisms capable of degrading lignin with a focus on bacteria was analyzed. The consortiums were obtained from three types of soil, two commercial soils and one garden compost soil, cultivated at 30˚C and enriched through successive passages in a culture medium in which the lignin extracted by the alkaline method was used as the only carbon source. The metagenomic DNA of the third pass of the enrichment was extracted, and sequencing by the Joint Genome Institute, generating 232 assembled genomes, highlighting 39 genomes after quality criteria of completeness of at least 70% and contamination of less than or equal to 10%. Of these 39 genomes, the most abundant phyla in the three consortia were proteobacteria, bacteroidetes and actinobacteria, with only two genomes having higher species taxonomy. The consortiums presented functions consistent with the place of isolation, in this case the soil, in addition to possible metabolisms related to lignin degradation. 4 genomes were chosen, based on taxonomy only, because they only reached the class level, for deeper analyzes of lignin degradation focused on monolignols. The genomes of Actinobacteria BY 70_11 and Actinobacteria BY 2_71_9 and Alphaproteobacteria MG 62_16 and Alphaproteobacteria MG 3_66_13, in addition to not representing the same species, showed different metabolic pathways related to the degradation of monolignols. Of these, Actinobacteria BY 70_11 had the highest number of genes associated with lignin degradation. However, it cannot be stated unequivocally about the ability or not to degrade the lignin encoded in these 4 genomes due to the lack of literature on these specific pathways. This work proved to be an initial step for others about lignin degradation with the same genomes with the expansion of metabolic pathways and with more genomes for deeper analyzes.

BANKING MEMBERS:
Externo à Instituição - FABYANO ALVARES CARDOSO LOPES - UFT
Externa à Instituição - JESSICA CARVALHO BERGMAN - EMBRAPA
Presidente - 1661623 - RICARDO HENRIQUE KRUGER
Interno - 2644635 - ROBERT NEIL GERARD MILLER