Banca de QUALIFICAÇÃO: Amanda Cristina de Araújo

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : Amanda Cristina de Araújo
DATE: 21/08/2023
TIME: 08:00
LOCAL: Auditório 03 do IB/UnB
TITLE:

TRANSCRIPTIONAL DYNAMICS IN TRANSGENIC PLANTS OVEREXPRESSING GENES RELATED TO PLANT IMMUNITY

KEY WORDS:

Gene expression, transcriptome, Defense genes, Plant immunity

PAGES: 120
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The significant effects of biotic and abiotic stresses on agricultural productivity exhibit variations depending on the specific type of crop, region, and year. These impacts trigger economic consequences of large magnitude for the agricultural sector and for the country's economy as a whole. To mitigate the devastating effects of such losses, caused by both biotic and abiotic stresses in agriculture, a prominent strategy is the use of biotechnological approaches for crop improvement. For genetic modification, it is necessary to identify molecular pathways that orchestrate the plant immune process. In this context, the prospecting and identification of defense genes originating from wild plant species is a relevant strategy, as these plants, due to their co-evolution with pathogens, are considered a reservoir of genetic diversity. Numerous genes play crucial roles in protecting plants against adverse environmental conditions. Such defense genes encompass those that protect against biotic stresses, such as R genes, which directly interact with Avr genes in pathogens, as well as genes that provide defense against abiotic stresses, safeguarding plants against hostile environmental conditions. The aim of this study was to identify and analyze the behavior of defense genes under different stress conditions using the model plant Arabidopsis thaliana. Through the employment of genetic transformation, phenotyping techniques, and transcript sequencing, we were able to observe the behavior of different categories of candidate genes when plants were exposed to damage caused by pathogens (Meloidogyne incognita, Fusarium oxysporum f. sp. conglutinans) and abiotic stresses (drought). In the various systems studied, overexpressed genes (AdEXP4 and NBS19) caused statistically significant transcriptional modifications in transgenic plants and altered the manner in which the model species responded to the applied stresses. The results obtained reveal in planta roles for the candidate genes that are important for plant genetic improvement approaches for increased resistance to biotic stresses and increased tolerance to abiotic stresses

COMMITTEE MEMBERS:
Externa à Instituição - ANGELA MEHTA DOS REIS - EMBRAPA
Presidente - 2222088 - ELIANE FERREIRA NORONHA
Externa à Instituição - PRISCILA GRYNBERG - EMBRAPA
Interno - 1661623 - RICARDO HENRIQUE KRUGER