Banca de DEFESA: Pedro Brício Brito Fernandes

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Pedro Brício Brito Fernandes
DATE: 16/09/2022
TIME: 09:00
LOCAL: Videoconferência
TITLE:

"Expression of Cry10Aa toxin in Solanum lycopersicum for resistance to tomato leafminer (Tuta absoluta)."

KEY WORDS:

Solanum lycopersicum, tomato, Micro-Tom, Agrobacterium, tomato leafminer, Tuta absoluta, Bacillus thuringiensis, Cry10Aa.

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

More than 90 genus are part of the Solanaceae family, with emphasis on the Solanum, considered one of the great branches of this taxonomic family with about 2,000 described species, distributed in tropical and subtropical regions of the world. The wide diversity of the genus Solanumrelates species with high nutritional, biotechnological, as well as medicinal value, among them, model plants for biotechnology, widely used in genomic and/or metabolic characterization studies, such as: the potato (Solanum tuberosum L.), eggplant (Solanum melongena), as well as tomato (Solanum lycopersicum). The tomato, in turn, is characterized by a fruit rich in vitamins and minerals, in addition to other important phytochemicals, standing out as one of the five largest commercial crops in the world, as well as for its biotechnological value, largely due to its lineage Micro-Tom, considered a model plant characterized by an optimized life cycle and to reduced physical stature, favoring its use in biotechnological investments. The susceptibility of tomato cultivation in the most diverse environmental conditions does not cancel out the damage caused by events of environmental instabilities, as well as by the continuous action of predators, a process that can reach the mark of up to 50% of total production losses in underdeveloped countries, being that, among the main predatory agents of tomato is the Traça-do-tomateiro (Tuta absoluta), native of South America and widely described as a generalized predator in different cultures, however, with a wide predatory target in tomato crops. In recent decades, frequent reports of T. Absoluta have been observed in regions not previously occupied by this pest, such as Asia, Africa, Europe and North America, with predatory damage caused by T. absoluta responsible for about 5% of total tomato losses worldwide. In turn, due to the challenges associated with the conventional control of T. absoluta, a process that limits its population expansion worldwide, the management strategies used, sometimes, are summarized in the repeated use of agrochemical agents, opening the way for environmental risks and public health associated with them. On the other hand, recent population monitoring studies have pointed to an increase in the emergence of pesticide resistance in populations of T. absoluta against the active ingredients commonly used in agriculture, emphasizing the need to search for alternative molecules with safety and efficacy. In this sense, among the main biomolecules of technological importance described in the last decades are the Bt protoxins, an integral part of the secondary metabolism of the bacterium Bacillus thuringiensis and holders of a vast literary content about their toxicity mediated by the dissolution of the toxin in intestinal pH in different ways insect larvae (pH 8.0). Among the widely studied Bt families are the Cry biomolecules, with high toxicity reported against diptera, coleoptera, nematode, as well as lepidoptera, and studies have frequently evidenced their cytotoxic capacity of these toxins against T. absoluta, with emphasis on α- Cry10Aa endotoxin, described in the literature with a focus on its toxicity against members of the Lepidoptera family, especially against moths. Based on these data and on the economic and cultural importance associated with the management of T. absoluta, one of the main predatory tomato pests in expansion worldwide, we propose here to obtain and validate the toxicity of a tomato carrying the cry10Aa transgene for the management of T. absoluta.

BANKING MEMBERS:
Interno - 1193623 - FERNANDO ARARIPE GONCALVES TORRES
Presidente - 308.609.971-20 - FRANCISCO JOSE LIMA ARAGAO - NÃO INFORMADO
Externo à Instituição - LEONARDO SILVA BOITEUX - EMBRAPA
Externo à Instituição - NATALIA FAUSTINO PIRES - EMBRAPA