Banca de DEFESA: JENIFFER MENDES DA SILVA FREIRE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JENIFFER MENDES DA SILVA FREIRE
DATE: 30/03/2026
TIME: 10:00
LOCAL: Universidade de Brasília
TITLE:

Transcriptomic analysis of quiescent human neutrophils and after dual stimulation with PAF and fMLP

KEY WORDS:

Neutrophils, Transcriptomics, Microarray, Dual stimulation, PAF and fMLP

PAGES: 52
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUMMARY:

Neutrophils are key cells of the innate immune system and exhibit remarkable functional plasticity in response to inflammatory microenvironmental cues. In this study, a transcriptomic analysis of quiescent human neutrophils and neutrophils subjected to dual stimulation with platelet-activating factor (PAF) and the chemotactic peptide N-formyl-methionyl-leucylphenylalanine (fMLP) was performed to investigate global gene expression changes associated with different activation states. Neutrophils isolated from healthy donors were analyzed under the experimental conditions using high-density microarray technology (Clariom S Human). The results demonstrated differential modulation of thousands of transcripts, including genes involved in cell migration, inflammatory signaling, metabolism, immune response, and cytoskeletal regulation. Distinct transcriptional profiles and gene clusters associated with dual stimulation were identified, suggesting the presence of integrated and intermediate functional states. These findings provide new insights into the molecular mechanisms underlying neutrophil activation under combined stimuli and highlight the complexity of innate immune responses.

COMMITTEE MEMBERS:
Externo à Instituição - AGENOR DE CASTRO MOREIRA DOS SANTOS JÚNIOR - HCB
Interna - 2862542 - KELLY GRACE MAGALHAES
Externo ao Programa - ***.367.691-** - LUCAS SILVA DE OLIVEIRA - UnB
Presidente - 1254630 - WAGNER FONTES