Banca de DEFESA: Andréia Camargo Pinheiro

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Andréia Camargo Pinheiro
DATE: 24/03/2023
TIME: 14:00
LOCAL: Auditoium 2 do IB – Instituto de Biologia
TITLE:

EVALUATION OF THE ANTI-INFLAMMATORY EFFECT OF PEQUI OIL-BASED NANOEMULSION (Caryocar brasiliense) ON in vitro MACROPHAGES AND in vivo EDEMA MODEL.

KEY WORDS:

pequi oil, nanotechnology, inflammation, nociception, macrophages, herbal medicines.

PAGES: 102
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Inflammation is a set of changes that occur in the body and begin immediately after a stimulus, remaining associated with various causes, ranging from abandonment to stress or dysfunction related to emotional, immunological or genetic factors. Chronic inflammation is related to several diseases, being related to the evolution of noncommunicable chronic diseases, such as obesity, diabetes and cancer, which are now the main causes of mortality in the world, a phenomenon that has contributed to a significant increase in the medical demand for inflammatory cases. Anti-inflammatory drugs are among the most prescribed and consumed drugs in the world, being used since 1897, with the production of aspirin. Despite being widely observed over these years, they still have an important limitation of use, which is associated with prolonged time and consequent adverse effects. New research has raised an alarming concern in relation to new discoveries relating the use of anti-inflammatories to gastrointestinal, cardiovascular, hepatic, renal, respiratory and pulmonary complications, which raises the need for new drugs with effective anti-inflammatory action and that bring safety for long-term use. In this regard, Brazilian biodiversity represents a rich source of release of bioactives that can contribute both to the resolution and prevention of inflammatory processes. Pequi oil (Caryocar brasiliense), a plant native to the Brazilian Cerrado, consists mainly of fatty acids and carotenoids that give it several biological properties, including an antiinflammatory effect. However, the hydrophobicity of these molecules represents a limit to their administration. Therefore, the pequi oil was nanostructured in the form of a nanoemulsion (PeNE) as a way to improve the transport and delivery of the oil to the cells. In this work, investigations were conducted in 3 chapters: (1) systematic review evaluating the anti-inflammatory potential of pequi oil in its free or nanostructured form; (2) synthesis and characterization of PeNE, evaluation of cytotoxicity in macrophages from the bone marrow of rats differentiated by L929, in vitro, on cell viability and evaluation of the anti-inflammatory potential on these cells regarding the modulation of inflammatory mediators and morphological activation changes; (3) synthesis, characterization and evaluation of the anti-inflammatory and nociceptive effect of PeNE by model of edema induced by carrageenan, in addition to toxicity evaluation through clinical, biochemical, hematological and histopathological analyzes in male rats Rattus novergicus, In vivo, in addition to evaluation of cytotoxicity, DNA fragmentation and incorporation of NePE in J774-1 macrophage cells, in vitro. The results showed that there are several studies indicating the anti-inflammatory activity of pequi oil, however, so far there is no systematic review involving these data and that the literature points to the interaction of pequi oil with mechanisms of the inflammatory process through the modulation of pro and anti-inflammatory mediators. In addition, success was achieved in obtaining stable PeNE and in accordance with the proposal to be used as a nanophytotherapy, with dose-dependent cytotoxic activity on macrophages, biocompatibility, anti-inflammatory and anti-nociceptive activity in in vitro and in vivo models. Thus, the use of nanostructured pequi oil as an alternative or complement to conventional anti-inflammatory drugs is promising and needs attention for future research studies to better understand its anti-inflammatory and anti-nociceptive potential.

BANKING MEMBERS:
Presidente - 1949262 - GRAZIELLA ANSELMO JOANITTI
Externa ao Programa - 2223020 - ANAMELIA LORENZETTI BOCCA
Externo ao Programa - 1652361 - MARCELO HENRIQUE SOUSA
Externa à Instituição - ANDREANNE GOMES VASCONCELOS - UDF
Externa à Instituição - PATRICIA BENTO DA SILVA - PERKINELMER