Banca de DEFESA: Bruna Mendonça Lima

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Bruna Mendonça Lima
DATE: 28/02/2023
TIME: 08:30
LOCAL: Embrapa Recursos Genéticos e Biotecnologia
TITLE:

Development and optimization of hybrid nanossystems for resveratrol transport through a membrane with porosity similar to the blood-brain barrier

KEY WORDS:

 Resveratrol, lipossomes, poloxamer, blood-brain barrier

PAGES: 78
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

 The central nervous system (CNS) is protected by a semipermeable dynamic structure, called the blood-brain barrier (BBB), which efficiently shields this area from possible changes caused by harmful agents from the environment. In this sense, liposomal nanosystems present gains in performance and functionality, with a view to facilitating the passage of drugs through this barrier. Among the nutraceuticals that can be therapeutically used, resveratrol (RES) is a very promising flavonoid for acting as an anti-inflammatory and antioxidant agent. In the present work, a liposomal phosphatidylcholine nanosystem was made associated with poloxamer (pluronic F127) micelles for RES encapsulation. Each step of the experiments (production of empty and RES-filled pluronic F127 micelles, empty and RES-filled liposomal nanosystems, empty and extruded extruded liposomal nanosystems with RES) were repeated at least three times to verify their reproducibility, in addition to having the diameter values hydrodynamic (Z-Ave and percentage numbers) and polydispersion (PdI) measurements accounted for. About the Z-Ave, each group obtained different results including statistical difference between then. Therefore was necessary to review the hydrodynamic diameter in numbers in order to solve this issue. The PdI showed the importance of the extrusion method to obtain a more monodisperse solution (close to 0,2). Afterwards, the encapsulation rate was evaluated in the three experiments (batches) of extruded liposomal nanosystems with RES with an encapsulation rate of 63.94%, 89.10% and 100%. Then, a new liposomal extruded structure was constructed with RES and subjected to passage through a membrane with porosity compatible with that of the BBB. This nanosystem was able to perform diapedesis and maintain its size close to before passage. The liposomal nanosystem proved to be effective for RES encapsulation with an encapsulation rate compatible with that of the reviewed literature when compared to other nanosystem types. The results confirm the initial assumptions of high efficiency of the encapsulation capacity of the in vitro analyses and in vivo future studies will be necessary to verify the functionality of RES delivery in animal models.  

BANKING MEMBERS:
Externa à Instituição - Juliana Junqueira Pinelli - EMBRAPA
Externo ao Programa - 1929494 - JOAO PAULO FIGUEIRO LONGO
Presidente - 804.882.851-00 - LUCIANO PAULINO DA SILVA - EMBRAPA
Externa à Instituição - VERA LÚCIA PERUSSI POLEZ - EMBRAPA