Banca de DEFESA: Julia Augusto Vieira

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Julia Augusto Vieira
DATE: 28/07/2023
TIME: 14:00
LOCAL: Meio virtual
TITLE:

Development of a nanobiosensor of gold nanoparticles (AuNPs) functionalized with anti-SARS-CoV-2 glycoprotein antibodies and testing in volunteer patients.

KEY WORDS:

SARS-CoV-2, gold nanoparticle, dynamic light scattering.

PAGES: 52
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

The current COVID-19 pandemic, caused by the SARS-CoV-2 virus, comprises an infectious disease. Coronaviruses (CoVs), which are part of a viral family, are recognized for causing a variety of respiratory diseases in humans, ranging from the common cold to rarer and more severe conditions such as Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). This information is recorded by the World Health Organization panel. As far as transmission is concerned, respiratory droplets are the primary means of contamination, allowing SARS-CoV-2 to be transmitted from an infected person to a healthy person through physical contact with the sick patient or by coming into contact with their belongings. Coronaviruses (CoVs) have the ability to remain on surfaces for several days, which may explain the case of SARS-CoV-2 and represent a prolonged risk of infection. Nanotechnology has found extensive application in various fields of study, including medicine, biolabeling, and agriculture. Nowadays, this technology has been employed in different domains of medical science, such as targeted drug delivery, cancer diagnosis, and the development of agents against viral, bacterial, and fungal infections. Gold nanoparticles (AuNPs) exhibit characteristics that include the ability to provide a stable surface for the immobilization of biomolecules, which is highly useful in the preparation of biosensors. They are considered the most stable metallic nanoparticles due to their optical, electronic, and catalytic properties, as well as having high biocompatibility and an improved rate of electron transfer. For these reasons, AuNPs have found wide applications in various types of biosensors. In the present study, the development of a nanobiosensor was conducted using AuNPs that were functionalized with antibodies specific for the SARS-CoV-2 glycoprotein. The main goal of this nanobiosensor was to enable highly specific detection of the SARS-CoV-2 virus. The characterization results of the naked and functionalized AuNP50 and AuNP100 showed that the nanoparticles were stable (hydrodynamic diameter and polydispersity index). The functionalized nanoparticles showed good performance regarding binding on SARS-CoV-2 viruses. Finally, when the functionalized AuNP100 were put in contact with the samples from positive patients, there was a change in the size of the nanoparticles verified by dynamic light scattering (DLS) technique.

COMMITTEE MEMBERS:
Externa à Instituição - Glêndara Aparecida de Souza Martins - UFT
Externa à Instituição - PATRICIA BENTO DA SILVA - UNESP
Presidente - 6404569 - RICARDO BENTES DE AZEVEDO
Interno - 1773830 - SACHA BRAUN CHAVES