Banca de DEFESA: Samuel Silva Morais

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Samuel Silva Morais
DATE: 08/12/2023
TIME: 14:30
LOCAL: Instituto de Ciências Biológicas – Auditório 2
TITLE:

"Pequi oil (Caryocar brasiliense) nanoemulsions: influence of the oil extraction method on cytotoxicity and anti-inflammatory activity in vitro".

KEY WORDS:

Caryocar brasiliense, nanoemulsion, anti-inflammatory, macrophage

PAGES: 40
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Inflammation is one of the body's defense processes and occurs in response to infections caused by microorganisms or tissue damage. Pequi oil extracted from Caryocar brasiliense found in the Cerrado region is popularly used for its anti-inflammatory and antioxidant properties due to the presence of fatty acids and carotenoids. Nanotechnology serves as a tool to overcome barriers in the administration of hydrophobic compounds. This study aimed to produce and evaluate the physico-chemical properties and cytotoxicity of nanoemulsions based in pequi oil (PeNE) obtained through different extraction methods: cold pressing, solvent (acetone), and boiling (artisanal method). The pequi oils were evaluated based on their lipid profile, acidity and peroxide values to ensure their quality after extraction processes. The nanoemulsions were formulated with pequi oil, egg lecithin (2:1 w/w), and PEG40 (2%) and dispersed in PBS by ultrasonication. The samples were evaluated using Dynamic Light Scattering (DLS) for 120 days at 4°C and 25°C to assess their stability, as well as pH and stress conditions (serial dilution from 0 to 1/128 and pH variation between 3-11). For cytotoxicity assays, RAW 264.7 macrophages were treated with different PeNE and had their viability analyzed by MTT assay. The pequi oils maintained high purity levels according to the evaluated indices. PeNE obtained with oil obtained by different extraction methods showed similar hydrodynamic diameter <100 nm, PDI <0.250, zeta potential between -1.5 and -2.5 mV, pH between 6 and 7, and remained stable during the dilution test without significant variation and pH variation tests showed zeta potential changes in the most acidic pH the nanoemulsions remained neutral (3) near to 0 mV the most basic (11) got negative near to -10 to -15 mV. The MTT assay indicated that the concentration of 360 µg/mL of different PeNE and free oils did not show significant cytotoxicity to the cells after 24 hours, with cellular viability >80%. The nanoemulsions formulated from pequi oil obtained by different extraction methods remained stable, and the extraction methods did not lead to differences between the samples in the evaluated parameters. These nanoemulsions showed promise for application with macrophages, which are crucial cells in the inflammatory process, demonstrating biocompatibility for future evaluations.

COMMITTEE MEMBERS:
Externo à Instituição - BRENO NORONHA MATOS - UNIEURO
Presidente - 1949262 - GRAZIELLA ANSELMO JOANITTI
Interno - 2107993 - LUIS ALEXANDRE MUEHLMANN
Interna - 2220195 - MONICA PEREIRA GARCIA