Banca de DEFESA: Mailson Alves Lopes

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Mailson Alves Lopes
DATE: 27/06/2024
TIME: 14:30
LOCAL: Auditório 2 - Faculdade de Ciências da Saúde
TITLE:

Evaluation of the dynamics of cfDNA release during surgery in patients with colorectal cancer

KEY WORDS:

colorectal cancer; cfDNA; surgery

PAGES: 67
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Approximately two-thirds of patients with colorectal cancer (CRC) undergo resection with curative intent; however, 30% to 50% of these patients experience recurrence. The concentration of cell-free DNA (cfDNA) before and after surgery may be related to the prognosis of patients with CRC, but there is limited information regarding cfDNA levels at the time of surgery. This study aimed to evaluate the dynamics of cfDNA release during surgery in patients with CRC. We analyzed surgical cfDNA release using plasma samples from 23 colorectal cancer patients at three key points during surgery: preoperative (immediately before surgery), intraoperative (during surgery), and postoperative (at the end of surgery). Automated electrophoresis was used to analyze cfDNA concentrations and fragment sizes, which were then correlated with clinical variables. Our findings indicate a significant increase in cfDNA release during and after surgery (3.2 and 2.3 folds higher respectively, p < 0.05). Characteristic fragments of cfDNA (<400 bp) predominated at all surgical stages; however, the release of genomic material (>400 bp) was also observed. Additionally, we found that cfDNA concentration increases intraoperatively in patients over 60 years old, patients with comorbidities, patients with CEA levels >5 ng/mL, and patients with locally advanced tumors (T3, T4, N-, M-).

COMMITTEE MEMBERS:
Externa ao Programa - 1562125 - ANDREA BARRETTO MOTOYAMA - UnBPresidente - 1643347 - FABIO PITTELLA SILVA
Externo à Instituição - JOAO NUNES DE MATOS NETO - CETTRO
Externo à Instituição - RINALDO WELLERSON PEREIRA - UCB