Banca de DEFESA: ANA LUCIA CARNEIRO SARMENTO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANA LUCIA CARNEIRO SARMENTO
DATE: 27/02/2023
TIME: 14:00
LOCAL: auditório da Pós-Graduação da Faculdade de Medicina
TITLE:

effect of transvaginal electrostimulation on tissues associated with the pelvic floor, vagina, urethra and rectum of multipparus and nuliparaus Wistar rats

KEY WORDS:

pelvic floor; urogenital hiatus; muscle strength; urinary incontinence; muscle weakness.

PAGES: 85
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

: Introduction: The pelvic floor (PF) formed by muscles and fascia acts in the maintenance of the urogenital hiatus, sustains and maintains the continence of Organs pelvic organs. Structural and/or functional changes resulting from parity and ageing, atrophy or muscle weakness can cause dysfunctions such as incontinence and prolapses. Treatment for these disorders is based on surgery for severe cases or conservative therapies such as physiotherapy. Electrostimulation (EE) is based on the application of intermittent low current electrical stimuli that evoke muscle contraction. Thus, this study evaluated the effect of transvaginal electrical stimulation (EV) on the histomorphometry of the PF musculature (MAPs) and tissues associated with the vagina, urethra and rectum of multiparous and nulliparous Wistar rats. Method: This is a descriptive, experimental and prospective longitudinal study carried out in 12-month-old multiparous and nulliparous Wistar rats (n = 5). For the EV (30 sessions) it was performed with an intravaginal probe with biphasic current, pulse width of 700-µs, frequency of 50 Hz and intensity of 2 mA. Morphometric data were obtained from epithelial, connective, and muscular tissues in pelvic histological specimens processed with routine techniques and stained with hematoxylin and eosin, Gomori's trichrome, and immunostaining with Ki67. Results: Results showed that: 1) increased the force of contraction of PFMs in nulliparous and multiparous; 2) increased thickness of MAPs (muscle hypertrophy) in nulliparous and multiparous by satellite cell mitosis and increased fiber diameter; 3) decreased the thickness of the lining epithelium in the vagina of nulliparous (CN > EVN) and increased in the urethra of multiparous (EVM > CM); 4) increased thickness of connective tissue associated with the three pelvic organs (vagina, urethra and rectum); 5) increased thickness of the muscular layer (smooth muscle) in the vagina and rectum of multiparous women, as well as in the urethra and rectum of nulliparous women; 6) increased the total number of leukocytes/200 µm2 ) in the mucous membranes of nulliparous and multiparous women; 7) increased the total number of fibroblasts/fibrocytes/700 mm2 ) in the mucous membranes of multiparous women, in comparison with the other groups (MC, NC, EVN); 8) increased the total number of cells (epithelial, fibroblasts, endothelial, satellite and smooth muscle) ki67+ , multiparous, compared to the control group (EVM > CM); 9) increased the total number of ki67+ satellite cells in nulliparous women compared to the control group (EVN > CN). Conclusion: The set of results demonstrate that the application of 30 sessions of EE with a pulse width of 700-µs, frequency of 50 Hz and intensity of 2 mA favored the structural gain, above all, in the connective and muscular tissues that reflected in the increase of muscle contraction strength. The results showed the effectiveness of endovaginal electrostimulation in nulliparous and multiparous rats, which may point to the effectiveness of its clinical use for strengthening the pelvic floor and favoring the continence of Organs pelvic organs

BANKING MEMBERS:
Externo ao Programa - 1646005 - AMILCAR SABINO DAMAZO
Externo à Instituição - LEONEL ALVES DE OLIVEIRA - ANVISA
Externa à Instituição - LETICIA MARTINS PAIVA - UNICEUB
Externa à Instituição - RENATA OLIVEIRA SOARES - FEPECS
Presidente - 3424061 - SELMA APARECIDA SOUZA KUCKELHAUS