Banca de DEFESA: Stefhani Barcelos

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Stefhani Barcelos
DATE: 30/06/2023
TIME: 09:00
LOCAL: Local: videoconferência. Plataforma Teams
TITLE:

: Evaluation of the regenerative potential of extracellular vesicles derived from bovine mesenchymal stem cells on ovarian stromal cells and in vivo ovarian injury model.

KEY WORDS:

Infertility; Assisted reproduction, in vitro embryo production, regenerative medicine, extracellular vesicles derived from mesenchymal stem cells, Ultracentrifugation; Ultrafiltration.

PAGES: 95
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

With an increase in infertility, there is a growing demand for assisted reproduction techniques and in vitro embryo production. However, specific treatments are required for the underlying causes of infertility. Mesenchymal stem cells (MSCs) have been used to treat ovarian dysfunction. Because part of the mechanism of action of MSCs is due to their paracrine action, in this study, we investigated the therapeutic potential of extracellular defeats derived from MSCs (MSC-EVs) resulting from ultrafiltration (UF) and ultracentrifugation (UC), evaluating their effects on bovine ovarian stromal cell migration and their use in an animal model of acute ovarian injury. MSCs were acquired from bovine adipose tissue and characterized according to the International Society for Cell Therapy guidelines. MSC-VEs were instructed on the secretome of these cells using UF and UC and evaluated for size, concentration, induction potential, and migration of bovine ovarian stromal cells using experiments and cell migration assays. After comparing the isolation methods, we evaluated the rate of viable oocyte retrieval and the number of embryos produced in cows treated with three different therapeutic regimens of MSCEVs in an acute injury model. Four follicular puncture sessions were performed for each group. The first group received only the vehicle after the first follicular puncture. In the second group, proteins produced by 7.5 × 106 MSCs were injected after the first follicular puncture. In the third group, fruit trees produced by 2.5 × 106 MSCs were injected after the first session, and in the fourth group, the same amount of vengeful was injected after sessions 1, 2, and 3. The characterization of the experimental experiments showed that there was no difference in particle size between the isolation methods, but UF showed a higher yield. Regardless of the method used, these possibilities were able to induce cell migration and anticipation. Considering that there was no difference between the methodologies in functional assays, the yield obtained through UF proved to be a determining factor for large-scale use in vivo. We evaluated three therapeutic regimens for holiday treatment, observed follicular punctures, and observed that the group treated with the least amount of insufficiencies in the three doses maintained oocyte production and increased in vitro embryo production. Therefore, MSC-EVs demonstrated beneficial effects on both cell and external migration in vitro and on vacation fertiertility with follicular puncture injury in vivo.

BANKING MEMBERS:
Externa ao Programa - 2374060 - CAROLINA MADEIRA LUCCI
Presidente - 3088161 - JULIANA LOTT DE CARVALHO
Externa à Instituição - PATRÍCIA FURTADO MALARD
Externo à Instituição - RINALDO WELLERSON PEREIRA