Banca de DEFESA: Madalena Maria Saldanha Coelho

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Madalena Maria Saldanha Coelho
DATE: 12/09/2022
TIME: 09:00
LOCAL: Plataforma Teams
TITLE:

Microbial characterization of the stages of the technological processing of the slaughter of swine from slaughterhouses located in the Federal District

KEY WORDS:

swine, slaughterhouses, Salmonella, Escherichia coli, Listeria monocytogenes, swine carcass

PAGES: 80
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Inspeção de Produtos de Origem Animal
SUMMARY:

The aim of this work was to carry out the microbiological characterization of swine carcasses from the Federal District region by counting Enterobacteriaceae, Escherichia coli and Total Coliforms, searching for Salmonella spp. and Listeria monocytogenes and to detect of virulence genes stx1, stx2 and eae for Escherichia coli, as well as antibiogram and verification of biofilm formation of the isolates. For this, swabs were collected at 5 points on the carcass (leg, loin, belly pork, armpit and jowls pork) and in 6 stages of the technological process of slaughter, being them after bleeding, after passing through the epilator, after manual toileting in the dirty area. , before and after evisceration and after the final carcass washing, before cooling the carcasses, in 2 slaughterhouses between September 2019 and July 2021. In the counts of Enterobacteriacea, Escherichia coli and total Coliforms, it was possible to identify the main contamination points in each slaughterhouse in the 3 collections carried out, being them after bleeding and after evisceration in the slaughterhouse A, after the manual toilet and after evisceration in the slaughterhouse B in the 1st collection, and in the 2nd collection the step after the evisceration for the 3 microorganisms. Escherichia coli were isolated in the two slaughterhouses and Salmonella spp. and Listeria monocytogenes only in slaughterhouse B. Antimicrobial multidrug resistance was detected in most E. coli isolates, with higher frequencies of resistance to amoxicillin, ampicillin, chloramphenicol, sulfonamides and streptomycin. The virulence genes eae, stx1 and stx2 were also detected in the E.coli isolates, with 3 isolates presenting the 3 genes together and antimicrobial multiresistance to at least 6 classes of antimicrobials. Furthermore, a high frequency of moderate and strong in vitro biofilm formation was detected in E.coli isolates. There was isolation of Salmonella spp. in the stage after bleeding in one of the slaughterhouses, presenting antimicrobial multiresistance to at least 3 classes of antimicrobials and weak ability to form biofilms or did not form biofilms in vitro. In this same slaughterhouse there was also the isolation of Listeria monocytogenes in the carcass. The isolates showed antimicrobial resistance to 4 tested antimicrobials, and were weak biofilm formers at temperatures of 10ºC, 24ºC and 37ºC, with a strong forming isolate at 37ºC. As this is the first study of microbial characterization in swine carcasses in DF, the identification of virulent strains of Escherichia coli, multiresistant with moderate to strong capacity to form biofilms, as well as the isolation of Salmonella spp. and Listeria monocytogenes with antimicrobial resistance and the ability to form biofilms demonstrate potential risks to public health.

BANKING MEMBERS:
Interna - 2324672 - ANGELA PATRICIA SANTANA
Interna - 1352451 - SIMONE PERECMANIS
Externo à Instituição - CRISTIANO SALES PRADO - UFG