Banca de DEFESA: Mikael Arrais Hodon

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Mikael Arrais Hodon
DATE: 28/04/2023
TIME: 14:00
LOCAL: Plataforma Teams
TITLE:

Evaluation of Three Diagnostic Tests for Bovine Brucellosis Surveillance in Tank Milk Samples

KEY WORDS:

Bovine brucellosis; herd test in milk from tanks; i-ELISA; milk ring test; qPCR; test validation; latent class models

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Saúde Animal (Programas Sanitários)
SUMMARY:

Bovine brucellosis is a zoonosis of great economic and public health impact. In Brazil, the National Program for the Control and Eradication of Brucellosis and Tuberculosis (PNCEBT) was created in 2001, with the specific objectives of reducing the prevalence and incidence of new outbreaks of brucellosis and tuberculosis and promoting the certification of properties free of brucellosis and tuberculosis and that would offer the consumer products of low health risk. After more than a decade of the creation of the PNCEBT, new strategies were devised, and it became necessary to implement active epidemiological surveillance systems to identify foci normally undetected by clinical signs or by routine control actions. In dairy herds, the tests applied to tank milk can also be appropriate for this purpose, provided that they are previously validated for the purpose for which they are intended. These tests have advantages such as low cost, ease of sample collection and speed in obtaining results. In the present study, the performance of three diagnostic tests for bovine brucellosis in tank milk samples from Santa Catarina (SC) were evaluated: milk ring test (TAL), the ELISA Brucellosis Antibody Test Kit – Tank Milk IDEXX (i-ELISA) and a qPCR developed by the LFDA-MG (PCR). A total of 187 tank milk samples were obtained from herds classified as Free (103) and from herds classified as Infected/Positive (84), all located in the state of Santa Catarina. The data obtained were tabulated and the parameters of interest, sensitivity and specificity of each test, were estimated by a Bayesian latent class model, for two populations and three diagnostic tests, two of them correlated - ELISA and TAL. The posterior estimates for the parameters were reported with 95% credibility. The i-ELISA yielded a sensitivity (Se) of 0.9 [0.73 - 0.99] and specificity (Sp) of 0.99 [0.95 – 1.0]. The TAL presented Se of 0.82 [0.68 – 0.93] and Sp of 0.84 [0.77 – 0.9]. Finally, the Se of qPCR was equal to 0.01 [0.0 – 0.05] and the Sp was 0.97 [0.93 – 0.99]. The results of this study indicate that i-ELISA would be the test of choice for surveillance of bovine brucellosis using samples from milk tanks, whereas TAL is a good alternative. The qPCR evaluated in this study would not be adequate as a tool for detecting infected herds.

BANKING MEMBERS:
Presidente - 2352263 - VITOR SALVADOR PICAO GONCALVES
Interno - 306996 - JOSE RENATO JUNQUEIRA BORGES
Externa à Instituição - ELAINE MARIA SELES DORNELES - UFLA
Externo à Instituição - MARCOS BRYAN HEINEMANN - USP