public health, synanthropic animals, infectious agents, felines.

Occurrences of domestic cats (Felis catus) inside and in the vicinity of Conservation Units (CUs) are common and associated with various issues. These animals can transmit zoonotic diseases and negatively impact the environment through predatory practices, putting endemic species of small mammals, birds, reptiles, and amphibians at risk. Moreover, the very lives of these animals may be in jeopardy as they are often mishandled and exposed to poisoning and slaughter. To minimize these impacts, it is recommended to carry out sanitary evaluations and proper management of domestic feline populations residing in CUs. Measures such as vaccination and neutering can be effective in the sanitary control of these animals. The objective of this study is to identify specific microorganisms in the population of domestic cats captured within the premises of the Brasília Zoo Foundation (FJZB) using Polymerase Chain Reaction (PCR) for Chlamydophila sp., feline herpesvirus, and Mycobacterium spp. Additionally, the animals underwent Kit-type tests, which simultaneously detect IgG antibodies of the feline immunodeficiency virus (FIV) and antigens (p27 antigens) of the feline leukemia virus (FeLV). Regarding the results, all samples showed non-reactivity for Chlamydophila sp., feline herpesvirus, FIV, and FeLV. However, 76.19% of the samples were reactive for the microorganism Mycobacterium spp., highlighting the importance of studies like this for public health.