Banca de DEFESA: AMANDA DE OLIVEIRA ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : AMANDA DE OLIVEIRA ALVES
DATE: 26/07/2023
TIME: 14:00
LOCAL: FAV
TITLE:

PATTERNS OF ANTIMICROBIAL RESISTANCE OF Staphylococcus aureus ISOLATED FROM BOVINE MASTITIS.

KEY WORDS:
PAGES: 52
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Staphylococcus aureus is commonly isolated in cases of subclinical mastitis in cattle, leading to significant damage to milk-producing tissues due to toxin release and resistance to treatment. Bovine mastitis poses a substantial economic impact on milk production, resulting in substantial losses for producers due to its complex and multifactorial nature, often involving various pathogens, notably staphylococci and streptococci bacterial groups. In the context of Brazil, bovine mastitis represents a relevant health issue, with production losses, animal disposal, and antibiotic usage potentially leading to residual effects in milk, compromising product quality and public health. This situation is associated with outbreaks of foodborne infections and the development of antimicrobial resistance. Antimicrobial resistance analysis indicated higher resistance to clarithromycin (100%), doxycycline (92.9%), linezolid (92.9%), and clindamycin (89.3%), and lower resistance to nitrofurantoin (10.7%), gentamicin (17.9%), vancomycin (17.9%), oxacillin (25%), cefotaxime (32.1%), ciprofloxacin (32.1%), and trimethoprim-sulfamethoxazole (39.3%). Nitrofurantoin exhibited the highest sensitivity profile, reaching 89.3%, followed by gentamicin and vancomycin reaching 82,1%. Regarding molecular identification, all strains were confirmed by the species-specific AroA gene. Amplified fragment sizes were observed for the CoA gene. The MecA gene was not detected in any sample, while the β-lactam resistance gene blaZ was present in 89.7% of samples. The tetK, tetM, vanA, and vanB genes showed positive reactions at 10/28 (34.5%), 10/28 (34.5%), 5/28 (17.2%), and 9/28 (31%) respectively. The results highlight a high profile of antimicrobial resistance to specific antibiotic classes and emphasize the importance of an accurate diagnosis to guide treatment decisions, given the microorganisms' genetic diversity and abundance of resistance genes.

COMMITTEE MEMBERS:
Presidente - 1352451 - SIMONE PERECMANIS
Interna - 2324672 - ANGELA PATRICIA SANTANA
Externa à Instituição - PAULA SUZANA ELISA MACIEL POLL