Characterization of Escherichia coli and Listeria monocytogenes isolates from slaughterhouse environments and foods of animal origin in Caco-2 cell culture
Listeria monocytogenes
Escherichia coli
antimicrobial resistance
Biofilms
The characterization of genotypic and phenotypic aspects of potentially pathogenic microorganisms, such as Escherichia coli and Listeria monocytogenes, originating from food and food production environments of animal origin, with the identification of the presence of virulence genes and the evaluation of cell adhesion patterns, verification of biofilm formation capacity and antimicrobial resistance research are tools that assist in surveillance to assess public health risk. In Brazil, there are few studies that promote the molecular characterization of these two microorganisms originating from food and food industry environments of animal origin. Therefore, the present project aims to research and characterize isolates of E. coli and L. monocytogenes from food and cold slaughterhouse environments for pigs, cattle and poultry in Caco-2 cell cultures and correlate them with the virulence of the isolates, as well as verifying the ability to form biofilms and researching the presence of phenotypic and genotypic antimicrobial resistance.