Banca de DEFESA: BRUNO HENRIQUE MONTEIRO LEITE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BRUNO HENRIQUE MONTEIRO LEITE
DATE: 23/06/2023
TIME: 16:00
LOCAL: On line (Plataforma Teams)
TITLE:

Development and validation of an analytical method using UHPLC-DAD for the determination of substituted N-benzyl phenethylamine

KEY WORDS:

25R-NBOH, 25R-NBOMe, UHPLC-DAD, Thermal Degradation, Hallucinogens, New Psychoactive Substances

PAGES: 76
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

The N-benzyl phenethylamine substituted, as 25R-NBOMe and 25R-NBOH compounds (R = Br, Cl, H, I, and ethyl), are potent serotonin receptor agonist (5- HT2A) detected in blotter papers worldwide. In forensic laboratories, those substances are analyzed using gas chromatography-mass spectrometry (GCMS) and liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). It is essential to develop a faster, simple, and sensitive method for the quantification of 25R-NBOH because this drug is a thermal labile compound. The objective of this study was to develop and validate a novel UHPLC-DAD method to determine 25R-NBOH in blotter papers. The analysis was performed in isocratic mode, using an Agilent 1290 liquid chromatography coupled to a diode array detector (UHPLC-DAD) was used for chromatographic separation. The mobile phase consisted of an acetonitrile/phosphate buffer 0.1 M (pH 2.3), in a proportion of 37:63. The method was validated over a concentration range of 200 ng/mL to 25,000 ng/mL. Both intra- and inter-assay precision and accuracy were < 6 % for all quality control samples. The development of a library containing UV spectra and relative retention time for LSD, 25R-NBOH, 25R-NBOMe and 2C-R compounds were also performed. The proposed method was evaluated in a routine analysis of 158 real samples and the mean concentration per blotter paper was determined for 25C-NBOH (237 ± 234 µg, n = 49), 25B-NBOH (900 ± 451 µg, n = 33), 25E-NBOH (819 ± 375 µg, n = 37) e 25I-NBOH (861 ± 383 µg, n = 28). Stability assays for the 25R-NBOH and 25R-NBOMe samples were prepared with methanol and water: acetonitrile (1:1) indicated that 25R-NBOH degradation occurs at 50 ºC during 50 days of storage. On the other hand, the long-term stability, at room temperature and in darkness, showed no 2C-R formation in blotter papers seized. The present assay is the first study to analyze those substances using UHPLC-DAD which is a faster and simpler method for sampling than previously published analytical reports for analyzing 25R-NBOH in blotter papers

BANKING MEMBERS:
Presidente - 1338848 - ANDERSON DE JESUS GOMES
Externa ao Programa - 2102264 - DAYANI GALATO
Externo ao Programa - 1742174 - JULIANO ALEXANDRE CHAKER
Externo à Instituição - JULIANO DE ANDRADE GOMES - PCDF