Banca de DEFESA: Isabella Possatti

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Isabella Possatti
DATE: 31/07/2023
TIME: 09:00
LOCAL: Plataforma Teams
TITLE:

Genetic variant DRD2 TaqIA in patients with Major Depressive Disorder

KEY WORDS:

DRD2; ANKK1; Major Depressive Disorder; DRD2TaqIA; rs1800497; Genetic Polymorphism.

PAGES: 145
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Introduction: Major Depressive Disorder is a disabling illness that affects millions of people annually. Its characteristic symptoms are lack of pleasure in carrying out daily activities and depressed mood for a period of 2 weeks or more. The monoamine theory is one of the most studied to try to explain the origin of depression and dopamine is inserted in this context, being responsible for motivation and the reward process when it binds to its receptors. One of them, the D2 receptor, is encoded by the DRD2 gene, which is located on chromosome 11q22. a thymine, consequently leading to a decrease in the bioavailability of dopamine D2 receptors. Objective: To analyze whether the DRD2TaqIA polymorphism is associated with Major Depressive Disorder through a scientific production in the form of a systematic review and from molecular analyzes in the laboratory. Methodology: The systematic review carried out followed the PECOS methodology, used to define the inclusion and exclusion criteria of the works. The search was carried out in 4 databases: PubMed, Web Of Science, Scopus and Virtual Health Library. Afterwards, the works were evaluated more specifically in the Rayyan tool and finally a table was created with the articles included. Molecular analyzes were performed from biological samples collected from patients with MDD who are followed up at the Psychosocial Care Center III, located in Samambaia Sul. DNA extraction was performed using the Polymerase Chain Reaction technique and samples were digested for genotyping using the TaqI restriction enzyme. Results: The systematic review obtained a final result of five articles and in two of them an association of the DRD2TaqIA variant with major depressive disorder and depressive symptoms was found, with the A1(T) allele being more present in the Chinese and American populations and the A2 allele (C) more present in the Russian population. Through molecular analysis, no statistical significance was found between the polymorphism studied in this work and major depressive disorder. Conclusion: The article revealed that the frequency of genotypes and alleles varies according to the population studied, but further studies are needed on this subject and the gene in question, for a better understanding of the impact of this polymorphism on the pathophysiology of depression.

COMMITTEE MEMBERS:
Externo à Instituição - BRUNO ROGERIO DE SOUZA - IFB
Interna - 1697332 - DANIELA CASTILHO ORSI
Presidente - 2495888 - IZABEL CRISTINA RODRIGUES DA SILVA
Externa à Instituição - MARIANA FURIO FRANCO BERNARDES - UNICESP