Banca de DEFESA: Giovanna Oliveira de Brito

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Giovanna Oliveira de Brito
DATE: 31/01/2023
TIME: 09:00
LOCAL: on line (Teams)
TITLE:

Microencapsulation and nanoencapsulation as strategies for the use of bioactives extracted from the peel of Spondias mombin L.

KEY WORDS:

cajá; teperebá; residue; stability; microencapsulation; nanoencapsulation; fruit from Cerrado; antitumor activity

PAGES: 100
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Introduction: Cancer is among the leading causes of death worldwide, and is therefore a major public health problem. The most frequent type of cancer in Brazil is skin cancer, with more than 180 thousand new cases, according to the National Cancer Institute (INCA) for 2016. Even though melanoma represents only 5% of all cases of this type of cancer, it has the highest mortality rate. Unhealthy eating patterns are among the leading risk factors associated with cancer incidence, which suggests that foods such as vegetables and fruits may regulate antitumor-related pathways. Among these bioactives, phenolic compounds are phytochemicals that have antioxidant action and can reduce the occurrence of various diseases. Studies indicate that, in biomes such as the Cerrado, the production of phytochemicals may be more significant, being a biome with representatives that demonstrate a high content of bioactives and antioxidant activity, such as cajá (Spondias mombin L.). However, the high reactivity of phenolic compounds makes them very unstable, which may compromise their beneficial properties. For this reason, microencapsulation or nanoencapsulation can protect these bioactives, maintaining their functions for longer. With this, in the scarcity of works on the subject, scientific investigation becomes important. Objectives: The objective of this work is to evaluate the viability of the nanoencapsulation process, and to evaluate the antitumor action of the skin cancer bark extract, more specifically melanoma. Materials and methods: The peel and pulp extracts were submitted to phenolic quantification. The nanoencapsulation was made from muru-muru butter, with buriti oil, and their characterization will be made. The nanoparticles will be evaluated for their stability in periods of 1, 7, 15, 30 and 60 days after preparation. Melanoma cancer cell lines (B16) were subjected to different concentrations of caja extract and microcapsules. Results: The ethanolic extract of cajá bark presented 457.90 mg/100g bark. The nanoencapsulation of the extracts, after their concentration in the rotary evaporator, obtained an encapsulation efficiency of 82.12% and nanoparticles increased the stability of the phenolic compounds. The nanocapsules were not able to induce B16 cancer cell death at the tested concentrations.

BANKING MEMBERS:
Externa à Instituição - LEILA DENISE FALCÃO
Interna - 1644041 - CLAURE NAIN LUNARDI GOMES
Presidente - 1804508 - ELIANA FORTES GRIS
Interno - 1652361 - MARCELO HENRIQUE SOUSA