Banca de DEFESA: Isolda de Sousa Monteiro

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Isolda de Sousa Monteiro
DATE: 04/12/2023
TIME: 09:00
LOCAL: PLATAFORMA TEAMS
TITLE:

Assessment of the wound-healing potential of andiroba (Carapa guianensis Aublet) based nanoemulsions in vitro.

KEY WORDS:

Carapa guianensis; wound healing; nanoemulsion, oil

PAGES: 51
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Healing is a complex process involving cellular, biochemical, and molecular mechanisms. Non-healing wounds result from a disruption in the physiological sequence of cellular and biochemical events responsible for restoring skin integrity. Andiroba oil (AO) is extracted from the seeds of Carapa guianensis Aublet and possesses therapeutic properties, including anti-inflammatory and wound healing effects. The use of nanoemulsions has shown promise in delivering and improving the bioavailability of hydrophobic bioactive compounds. The aim of this study was to develop a topical nanoemulsion based on AO and assess its wound healing potential in vitro. Commercially acquired AO was characterized for acidity, peroxide index, fatty acid profile, Fourier Transform Infrared Spectroscopy (FTIR), and Nuclear Magnetic Resonance (NMR). The nanoemulsion (NeAnd) was prepared with a 2:1 surfactant to-oil ratio (w/w) using ultrasonication. The cytotoxicity of NeAnd was tested on human keratinocytes (HaCat) using the MTT method. A scratch assay was performed to evaluate the effect of NeAnd on cell migration. Data were statistically analyzed using ANOVA and Tukey's post-test. The lipid profile confirmed the presence and expected proportion of fatty acids in andiroba oil. Additionally, bioactive compounds such as 2- undecenal, ethyl oleate, and ethyl palmitate were identified. FTIR revealed typical bands related to acyl chains of lipids, and NMR confirmed the major compounds in AO. NeAnd exhibited stability with an average hydrodynamic diameter of 205.7 ± 3.9 nm, polydispersity index (PdI) of 0.295 ± 0.05, zeta potential of -4.16 ± 0.41 mV, and a pH around 6.5. MTT assays showed that NeAnd and free andiroba oil at different concentrations (90, 180, and 360 μg/mL), only the AO group was cytotoxic at a concentration of 360 μg/mL (77.29%; p < 0.05) at 48 hours. In the scratch assay with keratinocytes, NeAnd (360 μg/mL) showed a significant increase (88.9%; p < 0.05) in cell migration, resulting in wound closure compared to the control group (PBS) and AO. These results demonstrate the biocompatibility and potential of the developed nanoemulsions in wound healing processes. Further studies in wound healing models are necessary for these formulations to be recognized as nano phytotherapeutics for clinical applications in wound healing and tissue regeneration.

COMMITTEE MEMBERS:
Externa à Instituição - LETÍCIA SCHERER KOESTER - UFRGS
Externo à Instituição - BRENO NORONHA MATOS - UNIEURO
Externo ao Programa - 1880131 - GUILHERME MARTINS GELFUSO - nullPresidente - 1707017 - MANI INDIANA FUNEZ