Banca de DEFESA: Jade Ormondes de Farias

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Jade Ormondes de Farias
DATE: 08/12/2022
TIME: 08:30
LOCAL: Plataforma Teams
TITLE:

Effects of senescence on the immune response of human pulp cells

KEY WORDS:

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PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Cell senescence is a biological response resulting from aging and chronic stress mainly characterized by a permanent state of cell cycle arrest. Evidence indicates that senescence can affect important functions of the dental pulp such as tissue defense and repair. This work evaluated possible morphological changes, in addition to the migratory and proliferative capacity and effects on the immune response of human dental pulp cells (CEP/UCB 4,714,331). Initially, the cells were treated with doxorubicin to induce senescence. Confirmation of induction was performed by β-galactosidase staining. Then, the morphological changes were evaluated through scanning electron microscopy, proliferation by counting cells not stained by trypan blue, and migration by the Scratch method. It was possible to observe morphologically an increase about cell size and a decrease in the number of cell extensions about morphology (p>0.03). Regarding proliferation capacity, a reduction of 36% at 24h and 35% at 48h (p<0.05) was observed, in addition to a decrease in migration capacity after senescence induction (p> 0.05). In this way, senescence was shown to be harmful to the proliferative and migratory capacity, in addition to affecting cell morphology

BANKING MEMBERS:
Presidente - 768.449.191-15 - TAIA MARIA BERTO REZENDE - UFMG
Externo ao Programa - 1752418 - JACY RIBEIRO DE CARVALHO JUNIOR
Externa à Instituição - ANA PAULA DIAS RIBEIRO - UFL
Externa à Instituição - FRANCINE BENETTI - UFMG