Banca de QUALIFICAÇÃO: Diogo de Amorim Barros

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : Diogo de Amorim Barros
DATE: 06/10/2023
TIME: 14:30
LOCAL: Auditório 1
TITLE:

IN VITRO EVALUATION OF THE ANTIFUNGAL ACTIVITY OF VAGINAL CAPULAE OF LYOPHILIZED AQUEOUS EXTRACT OF CERRADO PLANT SPECIES AND LACTOBACILLUS SPP. IN CLINICAL ISOLATES OF CANDIDA SPP

 

KEY WORDS:

Lactobacillus spp., Candida spp., Vulvovaginal candidiasis, vaginal capsule, plant extract

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Vulvovaginal candidiasis represents the second major cause of vaginitis after bacterial vaginosis and is diagnosed at least 1 time in life in 75% of women and 40-50% of them have the second episode. About 85% of vulvovaginal candidiasis are from C. albicans strains and the rest from non-albicans. About 5 to 15% of these are strains of C. glabrata. The main signs and symptoms associated with vulvovaginal candidiasis are severe vulvar itching, burning, leukorrhea, dyspareunia, dysuria, edema, vulvovaginal erythema, vulvovaginal fissures, odorless, thick, irregular vaginal discharge, similar to curd or rennet milk. Lactobacilli are the most numerous microorganisms in the vaginal microbiota and are commensal bacteria that help control strains of Candida spp. Plant extracts, through the chemical variability of their secondary metabolites, may also exhibit antifungal activity. Thus, the present study aimed to evaluate the microbicide activity of vaginal capsules of lyophilized aqueous extracts of Cerrado plants, as well as association with Lactobacillus rhamnosus and Lactobacillus crispatus in clinical isolates of Candida albicans 90028, Candida glabrata 90030 and Candida krusei ATTC 6258. For this, a series microdilution test was performed to determine the minimum inhibitory concentration of the extracts and in vitro assays of the vaginal capsules that presented pharmacotechnical feasibility. The lyophilized aqueous extracts of Morus nigra and Pouteria torta were analyzed alone at concentrations of 25 mg, 50 mg and 100 mg, or in association with Lactobacillus crispatus 1.0 x 109 CFU, or with Lactobacillus rhamnosus also at the same concentration, in mixed medium. After 12 hours in a water bath at 37 ºC, MRS Agar was plated in anaerobiosis or YM Agar medium and colony-forming units were counted. The experimental condition of highest microbicide activity for all clinical isolates of Candida spp. was the association of Pouteria torta 25 mg with Lactobacillus rhamnosus when plated in YM medium with an average percentage of fungal growth of 2.4%. Thus, it was concluded that among the lactobacilli evaluated, the one with the highest microbicide activity was L. rhamnosus. In addition, the association of Pouteria torta with L. rhamnosus promoted improvement of the microbicide effect, which may indicate synergistic activity. It is suggested to create a mathematical model that represents the optimization of these proportions from the multivariate linear regression technique. Then, analytical validation of the method to find, in a precise way, a formula that brings the ideal composition of the medication to the preformulation studies and subsequent clinical trials.

COMMITTEE MEMBERS:
Externo ao Programa - 1744566 - MAURICIO HOMEM DE MELLO - nullPresidente - 1562111 - PEROLA DE OLIVEIRA MAGALHAES DIAS BATISTA
Externa ao Programa - 2376979 - YANNA KARLA DE MEDEIROS NOBREGA - UnBInterna - 1844214 - YRIS MARIA FONSECA BAZZO