Banca de DEFESA: Katherine de Souza Rodrigues

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Katherine de Souza Rodrigues
DATE: 23/02/2024
TIME: 14:00
LOCAL: Plataforma Virtual - Microsoft Teams
TITLE:

Study of mutations related to the hTERT gene as potential risk or prognostic factors in breast cancer

KEY WORDS:

cancer; telomerase; polymorphism; hTERT; mutation

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Fisioterapia e Terapia Ocupacional
SUMMARY:

Changes in the promoter region of the telomerase reverse transcriptase (TERT) gene are significantly related to many types of cancer, and somatic mutations and single nucleotide polymorphisms (SNPs) in this region may influence cancer susceptibility and prognosis. Because telomerase is an important enzyme in the carcinogenesis process, because it is present in most tumors and because of the evidence that it is a good molecular marker, a deeper investigation into the subject is necessary. This study analyzed mutations in the telomerase promoter region in cancer patients and tested the correlation of such data with prognosis and various clinical variables. The biorepository had 221 patients and 18 types of cancer, the most frequent being breast, with 167 cases. Analyzes were performed from this group. Analyzing the different clinical associations, it is observed that high Ki67, negative ER and PR, Tumor Grade 3 and positive HER2 were associated with worse prognostic variables, confirming their clinical predictive values. As for the analysis of polymorphisms, 106 samples of patients with breast cancer were selected and 7 polymorphisms were analyzed: rs964200877, rs540807196, rs980822475, rs10078991, rs1054221410, rs930389112 and rs1433143099. Statistical analysis was performed for the rs10078991 polymorphism, as it was the only one to form the possibility of two groups with a considerable sample number: presence of Allele C (n=19) and absence of Allele C (n=79). The group with the TT genotype (Without Allele C) presented both favorable and unfavorable results for the prognosis of breast cancer, depending on the variable studied. Regarding a good prognosis, even larger tumors had less lymph node involvement. In contrast, the following variables were related to a worse prognosis: PR; BMI and T staging; and inflammatory infiltrate. The Allele C group showed a better relationship with a good breast cancer prognosis for the clinical variables: PR; BMI and N1 (Staging N); and inflammatory infiltrate. Despite the results of this polymorphism, when considering the analysis of the gene region, little variation between genotypes is observed.

COMMITTEE MEMBERS:
Externa à Instituição - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS - UFRN
Presidente - 1764941 - DIEGO MADUREIRA DE OLIVEIRA
Interno - 1127261 - RICARDO TITZE DE ALMEIDA
Externo ao Programa - 2001303 - RODRIGO HADDAD - nullExterna à Instituição - ROSÂNGELA VIEIRA DE ANDRADE - UCB