Banca de DEFESA: EVALINA COSTA DE SOUSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EVALINA COSTA DE SOUSA
DATE: 04/08/2023
TIME: 14:00
LOCAL: SALA DE REUNIÃO 04
TITLE:

Development of an innovative multifunctional device based on the essential oil of Syzygium aromaticum

KEY WORDS:

Syzygium aromaticum, microemulsion, acaricide, antimicrobial action product.

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Rhipicephalus (B.) microplus, popularly known as “carrapato-do-boi”, is the species that most compromises cattle breeding productivity in the country. Additionally, this parasite has a major negative impact on commercial, environmental, and public health. Nowadays, several strategies are available for controlling, preventing, and eliminating agricultural pests and other pathogenic microorganisms such as bacteria and fungi. In this way, applying formulations with innovative multifunctional devices based on essential oils (OEs) has been finding great efficiency in combating these etiological agents, mainly the Syzygium aromaticum essential oil (OESA). This work aims to obtain and characterize an innovative multifunctional device based on microemulsions containing the essential oil of S. aromaticum, dispersed in a saponeic base developed by a saponification reaction. It is expected that such system, when in contact with the aqueous medium, releases eugenol, an active ingredient of OESA and responsible for controlling agricultural pests due to an extensive acaricidal, bactericidal, and fungicidal action. The methodology was developed based on an experimental design study, including a pseudo-ternary phase diagram, the formulation selection, the development and physical-chemical characterization of the microemulsion, the production of a soap base, and the evaluation of acaricides and microbiological activity. The results obtained demonstrated that all three formulations remained with adequate viscosity, yellowish, clear, and translucent. Among the developed formulations, formulation 02 (ME25, 2:8:6 OESA: T/Cot: water) was chosen for the other tests due to its promising characteristics. The results indicated PDI values lower than 0.3, demonstrating an excellent homogeneity in the droplet diameter distribution. The zeta potential varied between -27.1 and - 39.9 mV. The selected formulation had a pH of 8.50. The soap base presented a semi-solid appearance, even after drying, compromising the tableting process. As for the acaricidal activity, using OESA, pure, and in the microemulsion significantly inhibited the posture of the engorged females, demonstrating an effectiveness of almost 100%. As for the larval forms, the group containing pure and microemulsified OESA resulted in mortalities above 99% and 95%, respectively. The microbiological evaluation showed an antimicrobial activity for both pure and microemulsified essential oil, classified as strong inhibitors, as they had a Minimum Inhibitory Concentration (MIC) of up to 500µg/mL. Thus, it is concluded that the microemulsion containing the essential oil of S. aromaticum was obtained satisfactorily, proving to be adequate in its physicochemical aspects. Furthermore, both the essential oil itself and the microemulsion showed capable of combating the species Rhipicephalus (B.) microplus, suggesting an excellent acaricidal activity, in addition to a satisfactory antimicrobial activity, requiring further research and experiments to elucidate the mechanisms of action of the compound.

COMMITTEE MEMBERS:
Externo à Instituição - DIEGO JUSCELINO SANTOS DIAS
Externa à Instituição - Heloísa Alves de Figueiredo Sousa
Externa ao Programa - 2495888 - IZABEL CRISTINA RODRIGUES DA SILVA - nullInterna - 1750177 - LIVIA CRISTINA LIRA DE SA BARRETO