Banca de QUALIFICAÇÃO: PAULA AKEMI ALBUQUERQUE KOMINAMI

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : PAULA AKEMI ALBUQUERQUE KOMINAMI
DATE: 23/08/2024
TIME: 08:30
LOCAL: Plataforma Teams
TITLE:

“Diversity and composition of biofilm associated with absence and presence of early childhood caries before and after treatment.”

KEY WORDS:

“Dental caries, child, deciduous tooth, microbiome. "

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

“_Introduction: Dental caries is a biofilm-mediated, sugar-dependent disease with a multifactorial and dynamic aetiology, in which cariogenic microorganisms play a fundamental role. The destruction of the biological balance in the biofilm and the disorganisation of this structure are closely linked to the occurrence and development of caries. A dysbiotic change in the oral microbiome begins before the manifestation of a caries lesion, making its characterisation challenging. Aim: The aim of this study was to assess the diversity of bacterial species in the dental biofilm of children with Early Childhood Caries before and after treatment. Methods: This is a longitudinal study nested within a birth cohort at the University Hospital of Brasilia. Children who returned for a 5-year follow-up and had at least one active or inactive caries lesion involving enamel or an active caries lesion involving dentin or a tooth without a caries lesion were included. Data on general and oral health was collected using a specific form. Treatment was carried out according to the type of caries lesion. To assess the microbiome in the oral cavity, biofilm was collected from children with IPC on the different surfaces of teeth without caries lesions, teeth with active and inactive enamel caries lesions and active cavitated caries lesions. The biofilm was collected before treatment (T0), after 66 months (T6) and 12 months (T12). Partial results: 50 children were included at baseline (mean age 66.94 months) and, at six months, 49 children came back. Of these, 55.1% were girls and 44.89% boys. At baseline, 33 patients had active lesions and 7 had inactive lesions. At 6 months, 13 patients who had active lesions at baseline returned without activity and 20 returned with new lesions on the same or different surfaces. Conclusion: The extraction, sequencing, analysis and bioinformatics of the DNA of the bacterial species found in the samples will be carried out in partnership with the Oral Biology Division of the Faculty of Dentistry at the University of Leeds, England.”

COMMITTEE MEMBERS:
Externa à Instituição - FERNANDA LOURENÇÃO BRIGHENTI - UNESP
Presidente - 2867208 - ELIANA MITSUE TAKESHITA NAKAGAWA
Interna - 3493627 - FERNANDA CRISTINA PIMENTEL GARCIA
Interna - ***.520.020-** - VANESSA POLINA PEREIRA DA COSTA - UFPel