Banca de DEFESA: Juliana Rodrigues e Silva

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Juliana Rodrigues e Silva
DATE: 28/07/2022
TIME: 08:30
LOCAL: Sala de reunião n° 5 (BANCA FECHADA)
TITLE:

Cytotoxicity in dental pulp cell culture and antimicrobial activity of chemically modified phenolic compounds.

KEY WORDS:

Eugenol, cytotoxicity, antimicrobial activity

PAGES: 1000
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Eugenol is a compound used for years in dentistry. It has interesting biological activities such as antifungal, antibacterial, antiparasitic, analgesic, anti-inflammatory, anticarcinogenic and antioxidant activities. It is used in cooking as an additive for food preservation. Despite these advantages, it has cytotoxic and genotoxic effects. The aim of this study was to evaluate the cytotoxicity and antimicrobial activity of different concentrations of methyl eugenol and ozonated methyl eugenol compared to eugenol in human dental pulp cell cultures. The study was divided into 3 chapters: In chapter 1, an introduction to eugenol bioactivities was presented. A literature review was also carried out with current research that contributed to the realization of this work and the general and specific objectives of this study were presented. In chapter 2, a study on the biocompatibility in human dental pulp cell culture was presented. The general objective of this work was to evaluate whether eugenol methylation andthe addition of ozone to the eugenol molecule would improve cytotoxicity in cells.
Experiments were performed to evaluate cell viability (MTT), expression of genes related to apoptosis (BAX, BCL2, CALPAIN, CASP3), by real-time PCR (qPCR). Cell death of cells exposed to the compounds was also qualitatively evaluated by light microscopy, after adaptation of Wright's colorimetric method. In chapter 3, a study was presented on the antimicrobial activity of phenolic compounds used in chapter 2 plus 3 phenolic compounds, guaiacol, methyl guaiacol and ozonized eugenol. The growth inhibition halo of microorganisms produced by phenolic compounds was evaluated in comparison with 2.5% sodium hypochlorite (2.5% NaOCl) in the bacterial strains Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus mutans. and in the fungus Candida albicans. Inhibition of growth of microorganisms exposed to the compounds
in planktonic form was evaluated in an automated way with Epoch 2 BioTek equipment.

BANKING MEMBERS:
Externo ao Programa - 1193623 - FERNANDO ARARIPE GONCALVES TORRES
Externa ao Programa - 3333449 - JANICE LISBOA DE MARCO
Externa à Instituição - LAIS FLAVIA NUNES LEMES
Presidente - 444.490.971-49 - LOISE PEDROSA SALLES - UNESP