"Biogeography of the Oral Archaeome"
Microbiota Oral; Domínio Archaea; biofilmes, biogeography cárie dentária, periodontite, infecções endodônticas, saliva
"There is evidence that archaea are part of the resident microbiota in various oral sites, such as periodontal and endodontic niches, carious dentin, saliva, and tongue, with current data suggesting that the abundance of methanogens increases along with the increase in the severity of periodontitis. More recently, the occurrence of archaea in carious dental tissue and supragingival biofilms has been reported. However, many questions regarding the prevalence and diversity of archaea in the oral cavity, pathogenic potential, and interactions with other oral microbial species remain undefined, in addition, new studies show that the global diversity of archaea in the human oral cavity may be underestimated and not restricted. to methanogenic organisms, as previously thought, so this cross-sectional study aims to map the occurrence of members of the Archaea domain in oral sites. The participants will be composed of patients recruited from the consultations carried out at the Oral Health Unit of the University Hospital of Brasília (HUB/UnB/Ebserh), who will donate a sample for the composition of one or more of the 7 research groups (Caries group; Biofilm group supragingival; Saliva group; Subgingivalbiofilm group in health; Subgingival biofilm group with periodontal disease; Lingual biofilm group and Endodontic biofilm group). The collected samples will be analyzed through RT PCR experiments; conventional PCR; Next generation sequencing (NGS) and fluorescence in situ hybridization (FISH)."