Banca de DEFESA: Isabela Monici Silva

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Isabela Monici Silva
DATE: 02/02/2024
TIME: 14:00
LOCAL: Plataforma Teams
TITLE:

Potential of cross-linking agents in the biomodification of root dentin

KEY WORDS:

Collagen; Dentin; Root caries; Hidroxyproline; In Vitro Techniques

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

There are several limitations associated with current treatments available for root caries, ranging from technical difficulties to cost-effectiveness. Hence, exploring the use of renewable sources for developing alternative techniques aimed at preserving dentin and its organic structure may potentially be applicable in clinical practice, as they represent biologically and economically feasible alternatives. One option could be the use of cross-linking agents, which by interacting with the collagen framework forming inter and intramolecular bonds, can improve the biochemical and mechanical properties of collagen present in dentin tissue in vitro, favoring not only the control of carious lesions but also remineralization. Dentin biomodification through cross-linking agents is already a highlighted subject in adhesion studies, considering the clinical need to promote greater longevity of the hybrid layer and consequently, of restorative treatments. Thus, this master's dissertation aimed to study the capacity of agents acting as cross-linkers in controlling proteolytic degradation and the formation of dentin carious lesions (Article I – Chapter 2), as well as investigating the interaction of saturated anacardic acid (LDT11), a potential natural cross-linker, with bovine root dentin (Article II – Chapter 3). Methodology: A systematic review of in vitro studies compared lesion size and changes in hydroxyproline release (biodegradation) in dentin samples subjected to cariogenic challenges after treatment with different cross-linking agents or without prior treatment. Additionally, Fourier-transform infrared spectroscopy in attenuated total reflection mode (FTIR-ATR) was used to verify the effect of the potential cross-linker agent (LDT11) with bovine dentin pretreated at a concentration of 100 µg/mL for 72 hours. Results: 50 articles were included, identifying 31 cross-linking agents. Out of these, 39 presented a low risk of bias, 10 a medium risk, and only 1 showed a high risk of bias. A positive effect of treatment with natural and synthetic crosslinkers on lesion size and biodegradation outcomes in dentin substrate samples exposed to cariogenic challenge was observed. Saturated anacardic acid, in turn, induced changes in the dentin's organic matrix suggestive of biomodification. Conclusion: Cross-linking agents seem to positively impact the control of in vitro dentin carious lesions, especially natural agents like Naringin, quercetin, and proanthocyanidins, as well as synthetic agents like glutaraldehyde. Clinical studies are necessary to assess the actual effectiveness and implications of these compounds on human root dentin, considering the oral environment's complexity due to its salivary, microbial, and pH composition. In this scenario, LDT11 might be a promising agent in controlling lesions and treatment longevity if its effectiveness in improving dentin's mechanical properties and increasing resistance to biodegradation is confirmed.

COMMITTEE MEMBERS:
Externa à Instituição - ALESSANDRA REIS - UEPG
Interna - ***.969.020-** - BRUNA GENARI DEGRAZIA - UFRGS
Presidente - 1306536 - NAILE DAME TEIXEIRA
Interna - 3249597 - RAYSSA FERREIRA ZANATTA