Banca de DEFESA: Michel Mattar Altoé

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Michel Mattar Altoé
DATE: 04/07/2023
TIME: 13:30
LOCAL: Plataforma Teams
TITLE:

“IDEATION, MODELING, 3D PRINTING AND IN VITRO EVALUATION OF BIOLOGICAL PROPERTIES OF A DENTIN DISC SUBSTITUTE.”

KEY WORDS:

"Three-dimensional printing, bioprinting, scaffold, structure, biomimicry, dentin disk."

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

“The use of human tissues, whether in surgical transplants or in research, presents several obstacles, highlighting among them the availability of donors and ethical aspects. 3D printing technology is currently emerging as a powerful tool in the effort to develop synthetic three-dimensional structures biologically like natural tissues for applications in various health fields. The objectives of this work were to carry out a literature review on the themes of 3D printing and bioprinting; production of a 3D print a biological substitute for the dentin disc; in vitro evaluation of the biological application on printed constructs. Human and animal dentin discs are the gold standard in research in dentistry, however, they are difficult to obtain, their use involves health, ethical and legal issues. In addition to preparing the discs from the teeth, they are quite laborious. The biggest challenge of this research was to develop structures with complex morphologies suchlike to natural tissue for use as a scaffold for human cells and in microbiological tests. The composition and morphology are fundamental to create a favorable environment for cell proliferation, extracellular matrix production and biofilm formation by microorganisms. The size and shape of the pores play a fundamental role that contribute to obtaining a functional interface between the cells and the biomaterial. In this study, software and a plugin were used, with a graphical interface of algorithms, which have parametric tools for modeling complex structures. The first chapter of this paper constitutes a literature review on 3D printing, bioprinting, definition, history, technological advances, materials, among others. The second chapter describes the process of ideation, planning and 3D printing of biomimetic structures to the dentin disc. The third chapter presents the microbiological and cell culture evaluation of the dentin disc substitute. Microscopic analysis of the construct demonstrated favorable results as a support for the growth of microbial biofilms. The SEM evaluations of the scaffolds, after cultivation with osteoblastic cells, demonstrated biocompatibility, cell adhesion to the surface and production of mineralized structures on the dentin disk substitute.”

BANKING MEMBERS:
Externo à Instituição - ALEXSANDRO SOBREIRA GALDINO - UFSJ
Interno - 4208835 - LAUDIMAR ALVES DE OLIVEIRA
Presidente - 444.490.971-49 - LOISE PEDROSA SALLES - UnB
Interna - 1306536 - NAILE DAME TEIXEIRA