Banca de QUALIFICAÇÃO: Caterynne Melo Kauffmann

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : Caterynne Melo Kauffmann
DATE: 29/09/2023
TIME: 14:00
LOCAL: Plataforma teams
TITLE:

"Study of infection and interaction of coguvirus and tobravirus".

KEY WORDS:

Transient expression, seed-transmission, subcellular localization, HR-like

PAGES: 36
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is an economically significant crop worldwide, being one of the most cultivated cucurbits in Brazil (Santos et al. 2011). Brazil is the 6th largest global producer (FAOSTAT 2020). The watermelon crinkle leaf-associated virus 1 and 2 (WCLaV-1 and -2), members of the Coguvirus genus, were recently reported in Brazil in a mixed infection with groundnut ringspot orthotospovirus (GRSV) (Maeda et al. 2021). The symptoms observed in watermelon plantations include yellowing, curling, and reduction of the leaf blade. The detection of viruses in field-collected plant materials is often performed using serological methods, as they are more accessible for testing numerous samples (Agrios, 2005) compared to reverse transcription polymerase chain reaction (RT-PCR). Given that WCLaV-1, WCLaV-2, and GRSV coexist in mixed infection, isolating each virus would be exceedingly complicated. Therefore, the preparation of recombinant N proteins from WCLaVs for antiserum production was planned. Our study was conducted to produce polyclonal antisera against WCLaV-1 and -2 using N proteins transiently expressed in plants through a vector. The serological characterization of the viruses involves detecting them in field plants, studying seed transmission, and performing immunolabeling. PepRSV is employed as a model for studying virus-plant interactions through infectious clones in our laboratory, as well as a plant viral vector for expressing recombinant proteins. To further enhance our understanding of this biotechnological tool, we will investigate the infectious clone of PepRSV with a mutation in the CP gene (clone 4) and the infectious clone of PepRSV with a knockout of a putative gene, referred to as ORFN1. Both clones induce local lesions similar to a hypersensitive reaction (HR-like) when agroinfiltrated into Nicotiana benthamiana plants. The objective of this study is to assess whether the absence of the putative ORFN1 from PepRSV and the mutation in clone 4 activate the endoplasmic reticulum stress pathway.

COMMITTEE MEMBERS:
Externa à Instituição - JULIANA DE FREITAS ASTÚA - EMBRAPA
Externa à Instituição - ALESSANDRA DE JESUS BOARI - EMBRAPA
Externa à Instituição - MARILIA SANTOS SILVA PATRIOTA - EMBRAPA
Externo à Instituição - RENATO DE OLIVEIRA RESENDE - OUTROS
Interno - 2644635 - ROBERT NEIL GERARD MILLER
Presidente - 1623558 - TATSUYA NAGATA