Banca de DEFESA: Alexandre Silva Santos

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : Alexandre Silva Santos
DATE: 01/06/2023
TIME: 09:00
LOCAL: Auditório do IF
TITLE:

Use of vibrational spectroscopy in the characterization of nanocarriers based on lipid and metallic nanoparticles loaded with bioactive molecules.

KEY WORDS:

FTIR; Raman; drug delivery system; nanostructured lipid; fish oil nanoemulsion; tris-(1,10-phenan-throline) iron(II); SARS-CoV-2

PAGES: 100
BIG AREA: Ciências Exatas e da Terra
AREA: Física
SUMMARY:

In this thesis, Raman spectroscopy and Fourier transform infrared (FTIR) techniques were used to study nanocarriers based on lipidic and metallic nanoparticles loaded with bioactive molecules. The temporal stability regarding the oxidation state of the nanoemulsion based on fish oil (FO), rich in docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) was evaluated over time after prepa-ration, at 0, 365 and 730 days. The FTIR data evidenced the presence of several chemical markers that showed a continuous oxidation of the FO nanoemulsion with the course of the preparation time. The results showed that due to their unsaturated characteristics, lipids are subject to molecular changes, which begin with the isomerization of double bonds cis to trans. These changes lead to oxidation processes and cleavages with the generation of by-products. The interaction between dif-ferent of [Fe(phen)3]2+ complex contents and solid lipid nanoparticles were investigated. The results showed the formation of a bidentate chelating bond between the carboxylate group from sodium ole-ate, present on lipid nanoparticle, and the Fe2+ ion of the [Fe(phen)3]2+ complex. Finally, the suc-cessful functionalization of the anti-SARS-CoV-2 antibody onto Au nanoparticles surface, precoated with 3,3'-dithiobis (Sulphosuccinimidyl Propionate-DTSSP) was demonstrated. The data showed that the primary amine groups of the antibody are covalently bound to the terminal succinimidyl ester group, forming a secondary amide bond by the nucleophilic attack of the amine to the ester carbonyl carbon present on the Au nanoparticle surface.

BANKING MEMBERS:
Externa à Instituição - ANIELLE CHRISTINE ALMEIDA SILVA - UFAL
Externa à Instituição - GUSTAVO OLIVEIRA DE MEIRA GUSMÃO - UESPI
Interno - 1999840 - JORLANDIO FRANCISCO FELIX
Interno - 1764408 - PAULO EDUARDO NARCIZO DE SOUZA
Presidente - 1210273 - SEBASTIAO WILLIAM DA SILVA