Banca de QUALIFICAÇÃO: OLGA MAIRA MACHADO RODRIGUES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : OLGA MAIRA MACHADO RODRIGUES
DATE: 22/12/2023
TIME: 14:00
LOCAL: Sala Teams do PPGMT
TITLE:

Genomic surveillance of tuberculosis and COVID-19 in the Federal District with transport of sample by drone.

KEY WORDS:

genomic surveillance, co-infection, tuberculosis, COVID-19.

PAGES: 70
BIG AREA: Ciências da Saúde
AREA: Medicina
SUBÁREA: Clínica Médica
SPECIALTY: Doenças Infecciosas e Parasitárias
SUMMARY:

Introduction: COVID-19 and tuberculosis (TB) are two infectious diseases considered serious threats to Public Health. There is scientific evidence that co-infection TB-COVID leads to worse outcomes. Objectives: Investigate cases of TB-COVID co-infection in the Federal District, diagnosed from 2021 to 2023, elucidating clinical, epidemiological and genomic aspects that may impact the outcomes, additionaly testing the transporto d respiratory samples by drone. Methods: This study contains epidemiological, genomic and innovation components. The database TB cases confirmed from 2021 and 2023 by Lacen-DF will be linked to that of COVID-19 to find cases of co-infection. The linkage will be carried out with Software R, using the variables name, date of birth and mother's name. Those eligible will be contacted by telephone and, later, in person, to obtain the informed consent form (ICF). When agreeing to participate, they will be included and investigated through electronic clinical records (in the Trakcare electronic medical record), epidemiological history (in SINAN and E-SUS VE) and laboratory records (Trakcare laboratory environment and GAL). Those who have viable isolates of Mycobacterium tuberculosis (Mtb) stored at Lacen-DF will be sequenced using the Illumina® method, seeking to identify, in comparison with the H37Rv strain, phylogenetic origin, genomic clusters, mutations related to drug resistance and single nucleotide polymorphisms (SNP) that may be related to COVID-19. The sequencing results will be analyzed through a Bioinformatics computational pipeline, involving filtering, mapping and annotation steps, using the Linux Operating System and R genomic sequencing software. The findings will be validated with the Fiocruz Genomic Network. Annotations will be checked against the main genomic libraries for Mtb. Additionally, sputum samples (positive for Mtb) and swab samples (positive for COVID-19) will be transported by drone, maintaining the appropriate biosafety and flight safety conditions, to test the feasibility of this transport, considering the quality of the samples (RT -PCR, rapid molecular test for tuberculosis and genomic sequencing). Partial results: The project has received Ethics Committee approval of FEPECS. The Lacen-DF Bacteriology Center has already shared the databases of TB cases from 2021 to 2022, as the 2023 is not yet complete. Initial stages of data curation were carried out, excluding duplicates and identifying 352 TB cases with samples stored at Lacen-DF. The next steps are to search for the cases’ mother's name and date of birth and link them to the COVID-19 database. For genomic analyses, materials and laboratory supplies are already being provided. The drone experiment will be held after obtaining the final list of eligible TB-COVID co-infected patients, with the appropriate ICS. A scoping review was carried out on the use of UAVs (unmanned aerial vehicles, better known as drones) in the transport of respiratory samples, and the article was submitted to the scientific journal Drones, where it is awaiting evaluation. Another (systematic) review of Mtb genomic sequencing is underway, seeking to identify the best methods for whole genomic sequencing of Mtb. 

COMMITTEE MEMBERS:
Presidente - 2646005 - AMILCAR SABINO DAMAZO
Interna - ***.580.701-** - TAINA RAIOL ALENCAR - UnB
Externo à Instituição - RENAN PEDRA DE SOUZA - UFMG