Banca de DEFESA: Juan Jorge Milla Espino

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Juan Jorge Milla Espino
DATE: 02/02/2024
TIME: 09:00
LOCAL: plataforma Teams NMT
TITLE:

ADENOSINE DEAMINASE ENZYME ACTIVITY IN PATIENTS WITH CUTANEOUS LEISHMANIASIS

KEY WORDS:

cutaneous leishmaniasis, adenosine deaminase, failure, diagnostic

PAGES: 70
BIG AREA: Ciências da Saúde
AREA: Medicina
SUBÁREA: Clínica Médica
SPECIALTY: Doenças Infecciosas e Parasitárias
SUMMARY:

Introduction: Leishmaniases are a group of infectious diseases caused by protozoa of the genus Leishmania. About 360 million people in 88 countries are at risk of developing some form of the disease. In 2022, 13,044 new cases of American Tegumentary Leishmaniasis were reported in Brazil. Cure criteria are clinical, based on complete re-epithelialization of the ulcers. There is a need to identify biological markers with high specificity and sensitivity that can be measured non-invasively to assist in patient monitoring and the evaluation of the therapeutic response objectively.

Methods: A study was conducted to assess the activity of the adenosine deaminase enzyme in patients diagnosed with cutaneous leishmaniasis. Fifteen patients newly diagnosed with cutaneous leishmaniasis were included and monitored monthly during and after treatment for a period of 90 days. At each assessment, a clinical evaluation was conducted, and venous blood was collected for subsequent evaluation of enzymatic levels. Concurrently, 10 participants were assessed in the control group only once. Enzymatic quantification was performed using the Adenosine Deaminase commercial kit from the Biotecnica company.

Results: In the study group, there was a predominance of males, married marital status, elementary education or lower, monthly income below 2 minimum wages, and the average age was 54.07 years. The control group showed a median value of adenosine deaminase of 6.29 U/L. In the study group, the values were 12.34 U/L at D0, 12.26 U/L at D30, 11.48 U/L at D60, and 8.62 U/L at D90. A statistically significant difference (p=0.001) was found between the control group and the study group values at D0. A significant difference was also found between the study group values at different follow-up points (p=0.004). Enzymatic activity was evaluated as a diagnostic test for cutaneous leishmaniasis using a cutoff value of 11.56 U/L, with a calculated specificity of 100%, sensitivity of 66.67%, and p=0.001. Enzymatic activity was also evaluated as a diagnostic test for therapeutic failure using a cutoff value of 14.13 U/

Conclusion : Analyses suggest a potential usefulness of enzyme levels as a diagnostic test for cutaneous leishmaniasis, but not as a diagnostic test for therapeutic failure. Some limitations of the study included a small sample size, the use of non-probabilistic sampling, and the non-identification of the Leishmania species. Studies with a larger sample and that include other clinical forms of the disease are suggested.

COMMITTEE MEMBERS:
Presidente - 2487183 - ELZA FERREIRA NORONHA
Interno - 2279074 - GUSTAVO ADOLFO SIERRA ROMERO
Interno - 3011946 - HENRY MAIA PEIXOTO
Externo à Instituição - JOSE ANGELO LAULETTA LINDOSO