Banca de DEFESA: Vitória França dos Santos Pessoa

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : Vitória França dos Santos Pessoa
DATE: 15/02/2024
TIME: 14:00
LOCAL: Auditório do Laboratório Interdisciplinar de Biociências - FM
TITLE:

Consequences of high-fat diet and physical training capacity in experimental Chagas disease

KEY WORDS:

Chagas disease, diet, experimental infection, physical exercise, prognosis

PAGES: 155
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is a serious challenge to public health, with significant morbidity and mortality, especially in Latin America. The pathogenesis is not well understood, and the immune response, as well as the clinical aspects of the disease, may be influenced by host habits such as dietary intake and physical activities. In fact, physical exercise (PE) may be a promising non-pharmacological alternative to improve host well-being and eventually for a better outcome of CD. This study aims to evaluate the long-term repercussions of a high-fat diet (HFD) and the capacity of moderate aerobic physical training in the acute phase of T. cruzi infection in female BALB/c mice. Furthermore, to verify the long-term consequences of this training conducted in the initial phase of infection. The animals were fed either a standard diet (SD) or HFD for 4 weeks, infected or not with T. cruzi, and trained or not on a treadmill for 6 weeks. Tests of maximal aerobic capacity (MAC) and strength were performed before, midway, and at the end of the training period. The dietary pattern was maintained throughout the experiment. After 90 days post-infection (dpi), the animals were euthanized. Serum cytokine levels were measured, parasite load quantified by qPCR, and histopathological analysis of different tissues was conducted. Infected mice were able to perform daily aerobic training at 60% of MAC. In the absence of training, muscle strength decreased with infection. HFD proved detrimental to muscle strength, even in uninfected mice. Likewise, this diet caused more inflammation in white adipose tissue (WAT), brown adipose tissue (BAT), and large intestine, especially in the presence of T. cruzi. HFD also increased parasite load in WAT and BAT. Physical training conducted in the acute phase of infection did not influence parasite load in the chronic phase and did not cause tissue alterations, except for BAT, which showed a higher degree of inflammation in trained mice. Given the variation in exercise adherence, the results of post-infection physical training require further investigation.

COMMITTEE MEMBERS:
Presidente - 2794682 - LUCIANA HAGSTROM BEX
Externo ao Programa - 2353151 - GUILHERME ECKHARDT MOLINA - UnBExterna ao Programa - 3365550 - KARINE BRENDA BARROS CORDEIRO - UnBExterna à Instituição - ANA MARIA DE CASTRO - UFG